2zg3

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[[Image:2zg3.jpg|left|200px]]<br /><applet load="2zg3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2zg3.jpg|left|200px]]
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caption="2zg3, resolution 3.0&Aring;" />
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'''Crystal Structure of Two N-terminal Domains of Native Siglec-5 in Complex with 3'-Sialyllactose'''<br />
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{{Structure
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|PDB= 2zg3 |SIZE=350|CAPTION= <scene name='initialview01'>2zg3</scene>, resolution 3.0&Aring;
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|SITE= <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+A+241'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+242'>AC2</scene> and <scene name='pdbsite=AC3:Bgc+Binding+Site+For+Residue+A+243'>AC3</scene>
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|LIGAND=
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|ACTIVITY=
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|GENE= SIGLEC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal Structure of Two N-terminal Domains of Native Siglec-5 in Complex with 3'-Sialyllactose'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ZG3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+A+241'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+242'>AC2</scene> and <scene name='pdbsite=AC3:Bgc+Binding+Site+For+Residue+A+243'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZG3 OCA].
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2ZG3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZG3 OCA].
==Reference==
==Reference==
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Structural implications of Siglec-5-mediated sialoglycan recognition., Zhuravleva MA, Trandem K, Sun PD, J Mol Biol. 2008 Jan 11;375(2):437-47. Epub 2007 Oct 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18022638 18022638]
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Structural implications of Siglec-5-mediated sialoglycan recognition., Zhuravleva MA, Trandem K, Sun PD, J Mol Biol. 2008 Jan 11;375(2):437-47. Epub 2007 Oct 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18022638 18022638]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: v-set]]
[[Category: v-set]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:01:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:08 2008''

Revision as of 16:54, 20 March 2008

Image:2zg3.jpg


PDB ID 2zg3

Drag the structure with the mouse to rotate
, resolution 3.0Å
Sites: , and
Gene: SIGLEC5 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Two N-terminal Domains of Native Siglec-5 in Complex with 3'-Sialyllactose


Overview

Sialic acid (Sia) Ig-like binding lectins are important mediators of recognition and signaling events among myeloid cells. To investigate the molecular mechanism underlying sialic acid Ig-like lectin (Siglec) functions, we determined the crystal structure of the two N-terminal extracellular domains of human myeloid cell inhibitory receptor Siglec-5 (CD170) and its complexes with two sialylated carbohydrates. The native structure revealed an unusual conformation of the CC' ligand specificity loop and a unique interdomain disulfide bond. The alpha(2,3)- and alpha(2,6)-sialyllactose complexed structures showed a conserved Sia recognition motif that involves both Arg124 and a portion of the G-strand in the V-set domain forming beta-sheet-like hydrogen bonds with the glycerol side chain of the Sia. Only few protein contacts to the subterminal sugars are observed and mediated by the highly variable GG' linker and CC' loop. These structural observations, in conjunction with surface plasmon resonance binding assays, provide mechanistic insights into linkage-dependent Siglec carbohydrate recognition and suggest that Siglec-5 and other CD33-related Siglec receptors are more promiscuous in sialoglycan recognition than previously understood.

About this Structure

2ZG3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural implications of Siglec-5-mediated sialoglycan recognition., Zhuravleva MA, Trandem K, Sun PD, J Mol Biol. 2008 Jan 11;375(2):437-47. Epub 2007 Oct 11. PMID:18022638

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