331d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:331d.gif|left|200px]]<br /><applet load="331d" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:331d.gif|left|200px]]
-
caption="331d, resolution 1.650&Aring;" />
+
 
-
'''CRYSTAL STRUCTURE OF D(GCGCGCG) WITH 5'-OVERHANG G'S'''<br />
+
{{Structure
 +
|PDB= 331d |SIZE=350|CAPTION= <scene name='initialview01'>331d</scene>, resolution 1.650&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''CRYSTAL STRUCTURE OF D(GCGCGCG) WITH 5'-OVERHANG G'S'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
331D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=331D OCA].
+
331D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=331D OCA].
==Reference==
==Reference==
-
Crystal structure of d(GCGCGCG) with 5'-overhang G residues., Pan B, Ban C, Wahl MC, Sundaralingam M, Biophys J. 1997 Sep;73(3):1553-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9284322 9284322]
+
Crystal structure of d(GCGCGCG) with 5'-overhang G residues., Pan B, Ban C, Wahl MC, Sundaralingam M, Biophys J. 1997 Sep;73(3):1553-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9284322 9284322]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Ban, C.]]
[[Category: Ban, C.]]
Line 22: Line 31:
[[Category: z-dna]]
[[Category: z-dna]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:04 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:40 2008''

Revision as of 16:54, 20 March 2008

Image:331d.gif


PDB ID 331d

Drag the structure with the mouse to rotate
, resolution 1.650Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF D(GCGCGCG) WITH 5'-OVERHANG G'S


Overview

The crystal structure of the DNA heptamer d(GCGCGCG) has been solved at 1.65 A resolution by the molecular replacement method and refined to an R-value of 0.184 for 3598 reflections. The heptamer forms a Z-DNA d(CGCGCG)2 with 5'-overhang G residues instead of an A-DNA d(GCGCGC)2 with 3'-overhang G residues. The overhang G residues from parallel strands of two adjacent duplexes form a trans reverse Hoogsteen G x G basepair that stacks on the six Z-DNA basepairs to produce a pseudocontinuous helix. The reverse Hoogsteen G x G basepair is unusual in that the displacement of one G base relative to the other allows them to participate in a bifurcated (G1)N2 . . . N7(G8) and an enhanced (G8)C8-H . . . O6(G1) hydrogen bond, in addition to the two usual hydrogen bonds. The 5'-overhang G residues are anti and C2'-endo while the 3'-terminal G residues are syn and C2'-endo. The conformations of both G residues are different from the syn/C3'-endo for the guanosine in a standard Z-DNA. The two cobalt hexammine ions bind to the phosphate groups in both GpC and CpG steps in Z(I) and Z(II) conformations. The water structure motif is similar to the other Z-DNA structures.

About this Structure

331D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Crystal structure of d(GCGCGCG) with 5'-overhang G residues., Pan B, Ban C, Wahl MC, Sundaralingam M, Biophys J. 1997 Sep;73(3):1553-61. PMID:9284322

Page seeded by OCA on Thu Mar 20 18:54:40 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/331d"
Personal tools