348d

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[[Image:348d.gif|left|200px]]<br /><applet load="348d" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:348d.gif|left|200px]]
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caption="348d, resolution 1.700&Aring;" />
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'''X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION'''<br />
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{{Structure
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|PDB= 348d |SIZE=350|CAPTION= <scene name='initialview01'>348d</scene>, resolution 1.700&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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348D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=348D OCA].
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348D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=348D OCA].
==Reference==
==Reference==
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X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9838004 9838004]
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X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9838004 9838004]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Finley, J B.]]
[[Category: Finley, J B.]]
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[[Category: double helix]]
[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:54:49 2008''

Revision as of 16:54, 20 March 2008

Image:348d.gif


PDB ID 348d

Drag the structure with the mouse to rotate
, resolution 1.700Å
Coordinates: save as pdb, mmCIF, xml



X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION


Overview

The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.

About this Structure

348D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:9838004

Page seeded by OCA on Thu Mar 20 18:54:49 2008

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