4b2o

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{{STRUCTURE_4b2o| PDB=4b2o | SCENE= }}
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==Crystal structure of Bacillus subtilis YmdB, a global regulator of late adaptive responses.==
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===Crystal structure of Bacillus subtilis YmdB, a global regulator of late adaptive responses.===
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<StructureSection load='4b2o' size='340' side='right' caption='[[4b2o]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24163345}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4b2o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B2O FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b2o RCSB], [http://www.ebi.ac.uk/pdbsum/4b2o PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacillus subtilis mutants lacking ymdB are unable to form biofilms, exhibit a strong overexpression of the flagellin gene hag, and are deficient in SlrR, a SinR antagonist. Here, we report the functional and structural characterization of YmdB, and we find that YmdB is a phosphodiesterase with activity against 2',3'- and 3',5'-cyclic nucleotide monophosphates. The structure of YmdB reveals that the enzyme adopts a conserved phosphodiesterase fold with a binuclear metal center. Mutagenesis of a catalytically crucial residue demonstrates that the enzymatic activity of YmdB is essential for biofilm formation. The deletion of ymdB affects the expression of more than 800 genes; the levels of the sigma(D)-dependent motility regulon and several sporulation genes are increased, and the levels of the SinR-repressed biofilm genes are decreased, confirming the role of YmdB in regulating late adaptive responses of B. subtilis.
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==About this Structure==
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The YmdB Phosphodiesterase Is a Global Regulator of Late Adaptive Responses in Bacillus subtilis.,Diethmaier C, Newman JA, Kovacs AT, Kaever V, Herzberg C, Rodrigues C, Boonstra M, Kuipers OP, Lewis RJ, Stulke J J Bacteriol. 2014 Jan;196(2):265-75. doi: 10.1128/JB.00826-13. Epub 2013 Oct 25. PMID:24163345<ref>PMID:24163345</ref>
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[[4b2o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B2O OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024163345</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Phosphodiesterase|Phosphodiesterase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacsu]]
[[Category: Bacsu]]
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[[Category: Diethmaier, C.]]
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[[Category: Diethmaier, C]]
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[[Category: Kovacs, A T.]]
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[[Category: Kovacs, A T]]
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[[Category: Kuipers, O P.]]
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[[Category: Kuipers, O P]]
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[[Category: Lewis, R J.]]
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[[Category: Lewis, R J]]
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[[Category: Newman, J A.]]
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[[Category: Newman, J A]]
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[[Category: Rodrigues, C.]]
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[[Category: Rodrigues, C]]
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[[Category: Stulke, J.]]
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[[Category: Stulke, J]]
[[Category: Biofilm]]
[[Category: Biofilm]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 14:22, 4 January 2015

Crystal structure of Bacillus subtilis YmdB, a global regulator of late adaptive responses.

4b2o, resolution 1.64Å

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