3b7b
From Proteopedia
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| - | [[Image:3b7b.jpg|left|200px]] | + | [[Image:3b7b.jpg|left|200px]] |
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| - | '''EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 1)''' | + | {{Structure |
| + | |PDB= 3b7b |SIZE=350|CAPTION= <scene name='initialview01'>3b7b</scene>, resolution 2.99Å | ||
| + | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+5'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+6'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+7'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+8'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+B+1'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+B+2'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+B+3'>AC7</scene> and <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+B+4'>AC8</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] | ||
| + | |GENE= EHMT1, EUHMTASE1, KIAA1876 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
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| + | '''EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 1)''' | ||
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| + | ==Overview== | ||
| + | Histone modifications have important roles in transcriptional control, mitosis and heterochromatin formation. G9a and G9a-like protein (GLP) are euchromatin-associated methyltransferases that repress transcription by mono- and dimethylating histone H3 at Lys9 (H3K9). Here we demonstrate that the ankyrin repeat domains of G9a and GLP bind with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9. X-ray crystallography revealed the basis for recognition of the methylated lysine by a partial hydrophobic cage with three tryptophans and one acidic residue. Substitution of key residues in the cage eliminated the H3 tail interaction. Hence, G9a and GLP contain a new type of methyllysine binding module (the ankyrin repeat domains) and are the first examples of protein (histone) methyltransferases harboring in a single polypeptide the activities that generate and read the same epigenetic mark. | ||
==About this Structure== | ==About this Structure== | ||
| - | 3B7B is a [ | + | 3B7B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B7B OCA]. |
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| + | ==Reference== | ||
| + | The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules., Collins RE, Northrop JP, Horton JR, Lee DY, Zhang X, Stallcup MR, Cheng X, Nat Struct Mol Biol. 2008 Mar;15(3):245-50. Epub 2008 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18264113 18264113] | ||
[[Category: Histone-lysine N-methyltransferase]] | [[Category: Histone-lysine N-methyltransferase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:56:46 2008'' |
Revision as of 16:56, 20 March 2008
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| , resolution 2.99Å | |||||||
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| Sites: | , , , , , , and | ||||||
| Ligands: | |||||||
| Gene: | EHMT1, EUHMTASE1, KIAA1876 (Homo sapiens) | ||||||
| Activity: | Histone-lysine N-methyltransferase, with EC number 2.1.1.43 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
EuHMT1 (Glp) Ankyrin Repeat Domain (Structure 1)
Overview
Histone modifications have important roles in transcriptional control, mitosis and heterochromatin formation. G9a and G9a-like protein (GLP) are euchromatin-associated methyltransferases that repress transcription by mono- and dimethylating histone H3 at Lys9 (H3K9). Here we demonstrate that the ankyrin repeat domains of G9a and GLP bind with strong preference to N-terminal H3 peptides containing mono- or dimethyl K9. X-ray crystallography revealed the basis for recognition of the methylated lysine by a partial hydrophobic cage with three tryptophans and one acidic residue. Substitution of key residues in the cage eliminated the H3 tail interaction. Hence, G9a and GLP contain a new type of methyllysine binding module (the ankyrin repeat domains) and are the first examples of protein (histone) methyltransferases harboring in a single polypeptide the activities that generate and read the same epigenetic mark.
About this Structure
3B7B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The ankyrin repeats of G9a and GLP histone methyltransferases are mono- and dimethyllysine binding modules., Collins RE, Northrop JP, Horton JR, Lee DY, Zhang X, Stallcup MR, Cheng X, Nat Struct Mol Biol. 2008 Mar;15(3):245-50. Epub 2008 Feb 10. PMID:18264113
Page seeded by OCA on Thu Mar 20 18:56:46 2008
Categories: Histone-lysine N-methyltransferase | Homo sapiens | Single protein | Cheng, X. | Collins, R E. | Horton, J R. | SO4 | Alternative splicing | Ank repeat | Ankyrin repeat | Chromatin regulator | Methyltransferase | Nucleus | Phosphorylation | Polymorphism | S-adenosyl-l-methionine | Transferase
