2bmy
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Banana lectin (Banlec) is a dimeric plant lectin from the jacalin-related, lectin family. Banlec belongs to a subgroup of this family that binds to, glucose/mannose, but is unique in recognizing internal alpha1,3 linkages, as well as beta1,3 linkages at the reducing termini. Here we present the, crystal structures of Banlec alone and with laminaribiose (LAM) (Glcbeta1, 3Glc) and Xyl-beta1,3-Man-alpha-O-Methyl. The structure of Banlec has a, beta-prism-I fold, similar to other family members, but differs from them, in its mode of sugar binding. The reducing unit of the sugar is inserted, into the binding site causing the second saccharide unit to be placed in, the opposite orientation compared with the other ligand-bound structures, of family members. More importantly, our structures . | + | Banana lectin (Banlec) is a dimeric plant lectin from the jacalin-related, lectin family. Banlec belongs to a subgroup of this family that binds to, glucose/mannose, but is unique in recognizing internal alpha1,3 linkages, as well as beta1,3 linkages at the reducing termini. Here we present the, crystal structures of Banlec alone and with laminaribiose (LAM) (Glcbeta1, 3Glc) and Xyl-beta1,3-Man-alpha-O-Methyl. The structure of Banlec has a, beta-prism-I fold, similar to other family members, but differs from them, in its mode of sugar binding. The reducing unit of the sugar is inserted, into the binding site causing the second saccharide unit to be placed in, the opposite orientation compared with the other ligand-bound structures, of family members. More importantly, our structures reveal the presence of, a second sugar binding site that has not been previously reported in the, literature. The residues involved in the second site are common to other, lectins in this family, potentially signaling a new group of, mannose-specific jacalin-related lectins (mJRL) with two sugar binding, sites. |
==About this Structure== | ==About this Structure== | ||
| - | 2BMY is a | + | 2BMY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Musa_acuminata Musa acuminata] with SO4 and CD as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BMY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: mannose-specific jacalin-related lectin]] | [[Category: mannose-specific jacalin-related lectin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:43:33 2007'' |
Revision as of 11:38, 5 November 2007
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BANANA LECTIN
Overview
Banana lectin (Banlec) is a dimeric plant lectin from the jacalin-related, lectin family. Banlec belongs to a subgroup of this family that binds to, glucose/mannose, but is unique in recognizing internal alpha1,3 linkages, as well as beta1,3 linkages at the reducing termini. Here we present the, crystal structures of Banlec alone and with laminaribiose (LAM) (Glcbeta1, 3Glc) and Xyl-beta1,3-Man-alpha-O-Methyl. The structure of Banlec has a, beta-prism-I fold, similar to other family members, but differs from them, in its mode of sugar binding. The reducing unit of the sugar is inserted, into the binding site causing the second saccharide unit to be placed in, the opposite orientation compared with the other ligand-bound structures, of family members. More importantly, our structures reveal the presence of, a second sugar binding site that has not been previously reported in the, literature. The residues involved in the second site are common to other, lectins in this family, potentially signaling a new group of, mannose-specific jacalin-related lectins (mJRL) with two sugar binding, sites.
About this Structure
2BMY is a Single protein structure of sequence from Musa acuminata with SO4 and CD as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Crystal structure of banana lectin reveals a novel second sugar binding site., Meagher JL, Winter HC, Ezell P, Goldstein IJ, Stuckey JA, Glycobiology. 2005 Oct;15(10):1033-42. Epub 2005 Jun 8. PMID:15944373
Page seeded by OCA on Mon Nov 5 13:43:33 2007
