3wnp

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{{STRUCTURE_3wnp| PDB=3wnp | SCENE= }}
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==D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose==
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===D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose===
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<StructureSection load='3wnp' size='340' side='right' caption='[[3wnp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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{{ABSTRACT_PUBMED_23908050}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3wnp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_24 Atcc 24]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WNP FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wnk|3wnk]], [[3wnl|3wnl]], [[3wnm|3wnm]], [[3wnn|3wnn]], [[3wno|3wno]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cit ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1397 ATCC 24])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wnp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wnp RCSB], [http://www.ebi.ac.uk/pdbsum/3wnp PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His(6) tag diffracted to a resolution of 2.3 A and belonged to space group P3(1)21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 A and belonged to space group P2(1)2(1)2(1), containing two molecules in the asymmetric unit.
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==Function==
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Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.,Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):946-9. doi:, 10.1107/S174430911301991X. Epub 2013 Jul 27. PMID:23908050<ref>PMID:23908050</ref>
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[[http://www.uniprot.org/uniprot/CTA1_BACCI CTA1_BACCI]] Produces cycloisomaltooligosaccharide from dextran containing 7, 8 or 9 glucose units. The enzyme is specific for (1->6)-alpha-D-glucans (dextrans) and, without activity toward (1->4)-alpha-D-glucans, such as amylose. It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points.<ref>PMID:7515357</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3wnp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WNP OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023908050</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
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[[Category: Atcc 24]]
[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
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[[Category: Fujimoto, Z.]]
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[[Category: Fujimoto, Z]]
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[[Category: Funane, K.]]
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[[Category: Funane, K]]
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[[Category: Kimura, A.]]
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[[Category: Kimura, A]]
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[[Category: Kimura, K.]]
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[[Category: Kimura, K]]
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[[Category: Kitamura, S.]]
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[[Category: Kitamura, S]]
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[[Category: Momma, M.]]
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[[Category: Momma, M]]
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[[Category: Suzuki, N.]]
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[[Category: Suzuki, N]]
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[[Category: Suzuki, R.]]
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[[Category: Suzuki, R]]
[[Category: 6-glucan]]
[[Category: 6-glucan]]
[[Category: Alpha-1]]
[[Category: Alpha-1]]

Revision as of 17:59, 21 December 2014

D308A, F268V, D469Y, A513V, and Y515S quintuple mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoundecaose

3wnp, resolution 2.80Å

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