3bjh
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:3bjh.jpg|left|200px]] | + | [[Image:3bjh.jpg|left|200px]] |
| - | + | ||
| - | '''Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.''' | + | {{Structure |
| + | |PDB= 3bjh |SIZE=350|CAPTION= <scene name='initialview01'>3bjh</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NBB:N-BUTYL-BENZENESULFONAMIDE'>NBB</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 3BJH is a [ | + | 3BJH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. This structure supersedes the now removed PDB entry 1R5R. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJH OCA]. |
==Reference== | ==Reference== | ||
| - | Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:[http:// | + | Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14594955 14594955] |
[[Category: Apis mellifera]] | [[Category: Apis mellifera]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 29: | Line 38: | ||
[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:59:32 2008'' |
Revision as of 16:59, 20 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Soft-SAD crystal structure of a pheromone binding protein from the honeybee Apis mellifera L.
Overview
Pheromone binding proteins (PBPs) are small helical proteins ( approximately 13-17 kDa) present in several sensory organs from moth and other insect species. They are involved in the transport of pheromones from the sensillar lymph to the olfactory receptors. We report here the crystal structure of a PBP (Amel-ASP1) originating from the honey-bee (Apis mellifera) antennae and expressed as recombinant protein in the yeast Pichia pastoris. Crystals of Amel-ASP1 were obtained at pH 5.5 using the nano-drops technique of crystallization with a novel optimization procedure, and the structure was solved initially with the single-wavelength anomalous diffraction technique using sulfur anomalous dispersion. The structure of Amel-ASP1 has been refined at 1.6-A resolution. Its fold is roughly similar to that of other PBP/odorant binding proteins, presenting six helices and three disulfide bridges. Contrary to the PBPs from Bombyx mori (Sandler, B. H., Nikonova, L., Leal, W. S., and Clardy, J. (2000) Chem. Biol. 7, 143-151) and Leucophea maderae (Lartigue, A., Gruez, A., Spinelli, S., Riviere, S., Brossut, R., Tegoni, M., and Cambillau, C. (2003) J. Biol. Chem. 278, 30213-30218), the extended C terminus folds into the protein and forms a wall of the internal hydrophobic cavity. Its backbone groups establish two hydrogen bonds with a serendipitous ligand, n-butyl-benzene-sulfonamide, an additive used in plastics. This mode of binding might, however, mimic that used by one of the pheromonal blend components and illustrates the binding versatility of PBPs.
About this Structure
3BJH is a Single protein structure of sequence from Apis mellifera. This structure supersedes the now removed PDB entry 1R5R. Full crystallographic information is available from OCA.
Reference
Sulfur single-wavelength anomalous diffraction crystal structure of a pheromone-binding protein from the honeybee Apis mellifera L., Lartigue A, Gruez A, Briand L, Blon F, Bezirard V, Walsh M, Pernollet JC, Tegoni M, Cambillau C, J Biol Chem. 2004 Feb 6;279(6):4459-64. Epub 2003 Oct 31. PMID:14594955
Page seeded by OCA on Thu Mar 20 18:59:32 2008
