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3dtj

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{{STRUCTURE_3dtj| PDB=3dtj | SCENE= }}
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==HIV-1 capsid C-terminal domain mutant (E187A)==
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===HIV-1 capsid C-terminal domain mutant (E187A)===
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<StructureSection load='3dtj' size='340' side='right' caption='[[3dtj]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_18772135}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3dtj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1 Hiv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DTJ FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ds4|3ds4]], [[3ds2|3ds2]], [[3ds3|3ds3]], [[3ds1|3ds1]], [[3ds5|3ds5]], [[3ds0|3ds0]], [[3dph|3dph]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11698 HIV-1])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dtj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dtj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dtj RCSB], [http://www.ebi.ac.uk/pdbsum/3dtj PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/3dtj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Morphogenesis of infectious HIV-1 involves budding of immature virions followed by proteolytic disassembly of the Gag protein shell and subsequent assembly of processed capsid proteins (CA) into the mature HIV-1 core. The dimeric interface between C-terminal domains of CA (C-CA) has been shown to be important for both immature and mature assemblies. We previously reported a CA-binding peptide (CAI) that blocks both assembly steps in vitro. The three-dimensional structure of the C-CA/CAI complex revealed an allosteric effect of CAI that alters the C-CA dimer interface. Based on this structure, we now investigated the phenotypes of mutations in the binding pocket. CA variants carrying mutations Y169A, L211A, or L211S had a reduced affinity for CAI and were unable to form mature-like particles in vitro. These mutations also blocked morphological conversion to mature virions in tissue culture and abolished infectivity. X-ray crystallographic analyses of the variant C-CA domains revealed that these alterations induced the same allosteric change at the dimer interface observed in the C-CA/CAI complex. These results point to a role of key interactions between conserved amino acids in the CAI binding pocket of C-CA in maintaining the correct conformation necessary for mature core assembly.
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==Function==
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Residues in the HIV-1 capsid assembly inhibitor binding site are essential for maintaining the assembly-competent quaternary structure of the capsid protein.,Bartonova V, Igonet S, Sticht J, Glass B, Habermann A, Vaney MC, Sehr P, Lewis J, Rey FA, Krausslich HG J Biol Chem. 2008 Nov 14;283(46):32024-33. Epub 2008 Sep 4. PMID:18772135<ref>PMID:18772135</ref>
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[[http://www.uniprot.org/uniprot/Q72497_9HIV1 Q72497_9HIV1]] Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3dtj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1 Hiv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DTJ OCA].
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</div>
==See Also==
==See Also==
*[[Virus coat protein|Virus coat protein]]
*[[Virus coat protein|Virus coat protein]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018772135</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Hiv-1]]
[[Category: Hiv-1]]
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[[Category: Igonet, S.]]
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[[Category: Igonet, S]]
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[[Category: Rey, F A.]]
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[[Category: Rey, F A]]
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[[Category: Vaney, M C.]]
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[[Category: Vaney, M C]]
[[Category: Assembly]]
[[Category: Assembly]]
[[Category: Capsid]]
[[Category: Capsid]]

Revision as of 18:05, 21 December 2014

HIV-1 capsid C-terminal domain mutant (E187A)

3dtj, resolution 4.00Å

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