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4kwb

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{{STRUCTURE_4kwb| PDB=4kwb | SCENE= }}
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==Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing and regulation==
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===Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing and regulation===
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<StructureSection load='4kwb' size='340' side='right' caption='[[4kwb]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24228759}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4kwb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KWB FirstGlance]. <br>
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==Function==
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bez|3bez]], [[3bfo|3bfo]], [[3rst|3rst]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU29530, CLONED SIGNAL PEPTIDE PEPTIDASE A GENE FROM RESIDUES 26 TO 335, LYS199 MUTATED TO ALA, sppA, yteI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kwb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kwb RCSB], [http://www.ebi.ac.uk/pdbsum/4kwb PDBsum]</span></td></tr>
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</table>
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== Function ==
[[http://www.uniprot.org/uniprot/SPPA_BACSU SPPA_BACSU]] Digestion of cleaved signal peptides (By similarity). Required for efficient processing of precursors under conditions of hyper-secretion.
[[http://www.uniprot.org/uniprot/SPPA_BACSU SPPA_BACSU]] Digestion of cleaved signal peptides (By similarity). Required for efficient processing of precursors under conditions of hyper-secretion.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial signal peptide peptidase A (SppA) is a membrane-bound enzyme that utilizes a serine/lysine catalytic dyad mechanism to cleave remnant signal peptides within the cellular membrane. Bacillus subtilis SppA (SppABS) oligomerizes into a homo-octameric dome-shaped complex with eight active sites, located at the interface between each protomer. In this study, we show that SppABS self-processes its own C-termini. We have determined the crystal structure of a proteolytically stable fragment of SppABSK199A that has its C-terminal peptide bound in each of the eight active sites, creating a perfect circle of peptides. Substrate specificity pockets S1, S3, and S2' are identified and accommodate C-terminal residues Tyr331, Met329, and Tyr333, respectively. Tyr331 at the P1 position is conserved among most Bacillus species. The structure reveals that the C-terminus binds within the substrate-binding grooves in an antiparallel beta-sheet fashion. We show, by C-terminal truncations, that the C-terminus is not essential for oligomeric assembly. Kinetic analysis shows that a synthetic peptide corresponding to the C-terminus of SppABS competes with a fluorometric peptide substrate for the SppABS active site. A model is proposed for how the C-termini of SppA may function in the regulation of this membrane-bound self-compartmentalized protease.
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==About this Structure==
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Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing, and regulation.,Nam SE, Paetzel M Biochemistry. 2013 Dec 10;52(49):8811-22. doi: 10.1021/bi4011489. Epub 2013 Nov, 25. PMID:24228759<ref>PMID:24228759</ref>
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[[4kwb]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KWB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024228759</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacsu]]
[[Category: Bacsu]]
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[[Category: Nam, S E.]]
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[[Category: Nam, S E]]
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[[Category: Paetzel, M.]]
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[[Category: Paetzel, M]]
[[Category: Alpha/beta protein fold]]
[[Category: Alpha/beta protein fold]]
[[Category: Bacterial cell membrane]]
[[Category: Bacterial cell membrane]]

Revision as of 21:13, 24 December 2014

Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing and regulation

4kwb, resolution 2.39Å

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