4c0h

Structural highlights

4c0h is a 4 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA.
Related: 4c0b
Activity: Glucokinase, with EC number 2.7.1.2

Publication Abstract from PubMed

Pcf11p and Clp1p form a heterodimer and are subunits of the Cleavage Factor IA (CF IA), a complex that is involved in the maturation of the 3'-end of mRNAs in Saccharomyces cerevisiae. The role of Clp1p protein in polyadenylation remains elusive, as does the need for ATP binding by Clp1p. In order to obtain structural details at atomic resolution of point mutants of Clp1p, we solved the crystal structure of Clp1-1p (G135R) point mutant complexed with Pcf11p (454-563) domain. The Clp1-1p-Pcf11p structure provides the atomic details for ATP loss while the point mutation preserves intact the Pcf11p interaction surface of Clp1p. This provides a rationale for the absence of phenotype in the yeast clp1-1 strain. Additionally, the structure allows for the description of an extended binding interface of Pcf11p with Clp1p which is likely to be S. cerevisiae specific.

Structural basis for ATP loss by Clp1p in a G135R mutant protein.,Dupin AF, Fribourg S Biochimie. 2014 Feb 5. pii: S0300-9084(14)00035-2. doi:, 10.1016/j.biochi.2014.01.017. PMID:24508575^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

1. ↑ Dupin AF, Fribourg S. Structural basis for ATP loss by Clp1p in a G135R mutant protein. Biochimie. 2014 Feb 5. pii: S0300-9084(14)00035-2. doi:, 10.1016/j.biochi.2014.01.017. PMID:24508575 doi:http://dx.doi.org/10.1016/j.biochi.2014.01.017