3no7
From Proteopedia
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| - | + | ==Crystal structure of the centromere-binding protein ParB from plasmid pCXC100== | |
| - | + | <StructureSection load='3no7' size='340' side='right' caption='[[3no7]], [[Resolution|resolution]] 1.40Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3no7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33973 Atcc 33973]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NO7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pCXC100 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31966 ATCC 33973])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3no7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3no7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3no7 RCSB], [http://www.ebi.ac.uk/pdbsum/3no7 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Plasmid pCXC100 from the Gram-positive bacterium Leifsonia xyli subsp. cynodontis uses a type Ib partition system that includes a centromere region, a Walker-type ATPase ParA and a centromere-binding protein ParB for stable segregation. However, ParB shows no detectable sequence homology to any DNA-binding motif. Here, we study the ParB centromere interaction by structural and biochemical approaches. The crystal structure of the C-terminal DNA-binding domain of ParB at 1.4 A resolution reveals a dimeric ribbon-helix-helix (RHH) motif, supporting the prevalence of RHH motif in centromere binding. Using hydroxyl radical footprinting and quantitative binding assays, we show that the centromere core comprises nine uninterrupted 9-nt direct repeats that can be successively bound by ParB dimers in a cooperative manner. However, the interaction of ParB with a single subsite requires 18 base pairs covering one immediate repeat as well as two halves of flanking repeats. Through mutagenesis, sequence specificity was determined for each position of an 18-bp subsite. These data suggest an unique centromere recognition mechanism by which the repeat sequence is jointly specified by adjacent ParB dimers bound to an overlapped region. | ||
| - | + | Crystal structure and centromere binding of the plasmid segregation protein ParB from pCXC100.,Huang L, Yin P, Zhu X, Zhang Y, Ye K Nucleic Acids Res. 2010 Dec 1. PMID:21123191<ref>PMID:21123191</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Atcc 33973]] | [[Category: Atcc 33973]] | ||
| - | [[Category: Ye, K | + | [[Category: Ye, K]] |
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Ribbon-helix-helix]] | [[Category: Ribbon-helix-helix]] | ||
Revision as of 21:30, 3 January 2015
Crystal structure of the centromere-binding protein ParB from plasmid pCXC100
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