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3wrv

From Proteopedia

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m (Protected "3wrv" [edit=sysop:move=sysop])
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'''Unreleased structure'''
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==Crystal structure of NN domain of resistance protein==
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<StructureSection load='3wrv' size='340' side='right' caption='[[3wrv]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3wrv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WRV FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vkw|3vkw]], [[3wrw|3wrw]], [[3wrx|3wrx]], [[3wry|3wry]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wrv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wrv RCSB], [http://www.ebi.ac.uk/pdbsum/3wrv PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tm-1, the protein product of Tm-1, a semidominant resistance gene of tomato, inhibits tomato mosaic virus (ToMV) replication by binding to ToMV replication proteins. Previous studies suggested the importance of the Tm-1 N-terminal region for its inhibitory activity; however, it has not been determined if the N-terminal region is sufficient for inhibition. Furthermore, the three-dimensional structure of Tm-1 has not been determined. In this study, an N-terminal fragment of Tm-1 (residues 1-431) as a fusion protein containing an upstream maltose-binding protein was expressed in E. coli Rosetta (DE3) cells at 30 degrees C and then purified. The solubility of the fusion protein was greater when the cells were cultured at 30 degrees C than when cultured at lower or higher temperatures. The purified N-terminal Tm-1 fragment from which the maltose-binding protein tag had been removed has inhibitory activity against ToMV RNA replication.
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The entry 3wrv is ON HOLD
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Expression, purification, and functional characterization of an N-terminal fragment of the tomato mosaic virus resistance protein Tm-1.,Kato M, Ishibashi K, Kobayashi C, Ishikawa M, Katoh E Protein Expr Purif. 2013 May;89(1):1-6. doi: 10.1016/j.pep.2013.02.001. Epub 2013, Feb 13. PMID:23415925<ref>PMID:23415925</ref>
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Authors: Matsumura, H., Katoh, E.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Crystal structure of NN domain of resistance protein
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Katoh, E.]]
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[[Category: Matsumura, H.]]
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[[Category: Alpha/beta domain]]
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[[Category: Resistance]]
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[[Category: Transferase]]

Revision as of 09:00, 13 August 2014

Crystal structure of NN domain of resistance protein

3wrv, resolution 2.75Å

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