473d
From Proteopedia
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| - | [[Image:473d.gif|left|200px]] | + | [[Image:473d.gif|left|200px]] |
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| - | '''NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2''' | + | {{Structure |
| + | |PDB= 473d |SIZE=350|CAPTION= <scene name='initialview01'>473d</scene>, resolution 1.58Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NT:NETROPSIN'>NT</scene> and <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 473D is a [ | + | 473D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=473D OCA]. |
==Reference== | ==Reference== | ||
| - | Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:[http:// | + | Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610787 10610787] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Abrescia, N G.A.]] | [[Category: Abrescia, N G.A.]] | ||
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[[Category: minor groove binding]] | [[Category: minor groove binding]] | ||
[[Category: netropsin/guanine stacking]] | [[Category: netropsin/guanine stacking]] | ||
| - | [[Category: ni2+/guanine | + | [[Category: ni2+/guanine interaction]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:08:40 2008'' |
Revision as of 17:08, 20 March 2008
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| , resolution 1.58Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2
Overview
We present the structure of the decanucleotide d(CGTATATACG) determined by single crystal X-ray diffraction at 1.58 A resolution. A netropsin drug is found in the minor groove with guanine stacked on a pyrrole ring of the drug, a feature described here for the first time. The stacked guanine is an extra-helical base coming from the end of a neighbour oligonucleotide. This observation may open the way to the development of minor groove binding drugs with a higher sequence selectivity. The oligonucleotide is in the B-conformation, but the terminal base-pairs are disrupted: the cytosine residues are disordered while the guanine residues penetrate into the minor groove of neighbouring duplexes. Four hydrated Ni ions with octahedral co-ordination are found associated with the N7 atoms of each guanine. The high affinity of these ions with guanine suggests that they may be used as probes for specific guanine residues.
About this Structure
473D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:10610787
Page seeded by OCA on Thu Mar 20 19:08:40 2008
