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4ou7

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'''Unreleased structure'''
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==Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode==
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<StructureSection load='4ou7' size='340' side='right' caption='[[4ou7]], [[Resolution|resolution]] 2.83&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ou7]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OU7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OU7 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ou6|4ou6]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ou7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ou7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ou7 RCSB], [http://www.ebi.ac.uk/pdbsum/4ou7 PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DnaT is a primosomal protein that is required for the stalled replication fork restart in Escherichia coli. As an adapter, DnaT mediates the PriA-PriB-ssDNA ternary complex and the DnaB/C complex. However, the fundamental function of DnaT during PriA-dependent primosome assembly is still a black box. Here, we report the 2.83 A DnaT84-153-dT10 ssDNA complex structure, which reveals a novel three-helix bundle single-stranded DNA binding mode. Based on binding assays and negative-staining electron microscopy results, we found that DnaT can bind to phiX 174 ssDNA to form nucleoprotein filaments for the first time, which indicates that DnaT might function as a scaffold protein during the PriA-dependent primosome assembly. In combination with biochemical analysis, we propose a cooperative mechanism for the binding of DnaT to ssDNA and a possible model for the assembly of PriA-PriB-ssDNA-DnaT complex that sheds light on the function of DnaT during the primosome assembly and stalled replication fork restart. This report presents the first structure of the DnaT C-terminal complex with ssDNA and a novel model that explains the interactions between the three-helix bundle and ssDNA.
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The entry 4ou7 is ON HOLD until Paper Publication
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Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode.,Liu Z, Chen P, Wang X, Cai G, Niu L, Teng M, Li X Nucleic Acids Res. 2014 Jul 22. pii: gku633. PMID:25053836<ref>PMID:25053836</ref>
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Authors: Liu, Z., Chen, P., Niu, L., Teng, M., Li, X.
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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Description: Structure of protein i form 2
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chen, P.]]
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[[Category: Li, X.]]
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[[Category: Liu, Z.]]
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[[Category: Niu, L.]]
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[[Category: Teng, M.]]
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[[Category: Dna binding]]
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[[Category: Replication-dna complex]]

Revision as of 08:57, 13 August 2014

Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode

4ou7, resolution 2.83Å

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