4cro
From Proteopedia
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| - | [[Image:4cro.gif|left|200px]] | + | [[Image:4cro.gif|left|200px]] |
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| - | '''PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX''' | + | {{Structure |
| + | |PDB= 4cro |SIZE=350|CAPTION= <scene name='initialview01'>4cro</scene>, resolution 3.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4CRO is a [ | + | 4CRO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CRO OCA]. |
==Reference== | ==Reference== | ||
| - | Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:[http:// | + | Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2146682 2146682] |
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:09:17 2008'' |
Revision as of 17:09, 20 March 2008
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| , resolution 3.90Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX
Overview
The structure of a complex of bacteriophage lambda Cro protein with a 17-base-pair operator has been determined at 3.9-A resolution. Isomorphous derivatives obtained by the synthesis of site-specific iodinated DNA oligomers were of critical importance in solving the structure. The crystal structure contains three independent Cro-operator complexes that have very similar, although not necessarily identical, conformations. In the complex, the protein dimer undergoes a large conformational change relative to the crystal structure of the free protein. One monomer rotates by about 40 degrees relative to the other, this being accomplished primarily by a twisting of the two beta-sheet strands that connect one monomer with the other. In the complex, the DNA is bent by about 40 degrees into the shape of a boomerang but maintains essentially Watson-Crick B-form. In contrast to other known protein-DNA complexes, the DNA is not stacked end-to-end. The structure confirms the general features of the model previously proposed for the interaction of Cro with DNA.
About this Structure
4CRO is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
Protein-DNA conformational changes in the crystal structure of a lambda Cro-operator complex., Brennan RG, Roderick SL, Takeda Y, Matthews BW, Proc Natl Acad Sci U S A. 1990 Oct;87(20):8165-9. PMID:2146682
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