4dmr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:4dmr.jpg|left|200px]]<br /><applet load="4dmr" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:4dmr.jpg|left|200px]]
-
caption="4dmr, resolution 1.90&Aring;" />
+
 
-
'''REDUCED DMSO REDUCTASE FROM RHODOBACTER CAPSULATUS WITH BOUND DMSO SUBSTRATE'''<br />
+
{{Structure
 +
|PDB= 4dmr |SIZE=350|CAPTION= <scene name='initialview01'>4dmr</scene>, resolution 1.90&Aring;
 +
|SITE= <scene name='pdbsite=MO:The+Substrate+Dmso+Is+Bound+At+The+Active+Site+To+The+Mo+...'>MO</scene>
 +
|LIGAND= <scene name='pdbligand=PGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>PGD</scene>, <scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene> and <scene name='pdbligand=DMS:DIMETHYL SULFOXIDE'>DMS</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''REDUCED DMSO REDUCTASE FROM RHODOBACTER CAPSULATUS WITH BOUND DMSO SUBSTRATE'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
4DMR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus] with <scene name='pdbligand=PGD:'>PGD</scene>, <scene name='pdbligand=4MO:'>4MO</scene>, <scene name='pdbligand=O:'>O</scene> and <scene name='pdbligand=DMS:'>DMS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MO:The+Substrate+Dmso+Is+Bound+At+The+Active+Site+To+The+Mo+...'>MO</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DMR OCA].
+
4DMR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_capsulatus Rhodobacter capsulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DMR OCA].
==Reference==
==Reference==
-
The high resolution crystal structure of DMSO reductase in complex with DMSO., McAlpine AS, McEwan AG, Bailey S, J Mol Biol. 1998 Jan 30;275(4):613-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9466935 9466935]
+
The high resolution crystal structure of DMSO reductase in complex with DMSO., McAlpine AS, McEwan AG, Bailey S, J Mol Biol. 1998 Jan 30;275(4):613-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9466935 9466935]
[[Category: Rhodobacter capsulatus]]
[[Category: Rhodobacter capsulatus]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 24: Line 33:
[[Category: substrate bound]]
[[Category: substrate bound]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:13:06 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:09:29 2008''

Revision as of 17:09, 20 March 2008

Image:4dmr.jpg


PDB ID 4dmr

Drag the structure with the mouse to rotate
, resolution 1.90Å
Sites:
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



REDUCED DMSO REDUCTASE FROM RHODOBACTER CAPSULATUS WITH BOUND DMSO SUBSTRATE


Overview

The crystal structure of the molybdenum enzyme dimethylsulphoxide reductase (DMSOR) has been determined at 1.9 A resolution with substrate bound at the active site. DMSOR is an oxotransferase which catalyses the reduction of dimethylsulphoxide (DMSO) to dimethylsulphide (DMS) in a two stage reaction which is linked to oxygen atom transfer and electron transfer. In the first step, DMSO binds to reduced (Mo(IV)) enzyme, the enzyme is oxidised to Mo(VI) with an extra oxygen ligand and DMS is released. Regeneration of reduced enzyme is achieved by transfer of two electrons, successively from a specific cytochrome, and release of the oxygen as water. The enzyme, purified under aerobic conditions, is in the oxidised (Mo(VI)) state. Addition of a large excess of DMS to the oxidised enzyme in solution causes a change in the absorption spectrum of the enzyme. The same reaction occurs within crystals of the enzyme and the crystal structure reveals a complex with DMSO bound to the molybdenum via its oxygen atom. X-ray edge data indicate that the metal is in the Mo(IV) state. The DMSO ligand replaces one of the two oxo groups which ligate the oxidised form of the enzyme, suggesting very strongly that this is the oxygen which is transferred during catalysis. Residues 384 to 390, disordered in the oxidised enzyme, are now clearly seen in the cleft leading to the active site. The side-chain of Trp388 forms a lid trapping the substrate/product.

About this Structure

4DMR is a Single protein structure of sequence from Rhodobacter capsulatus. Full crystallographic information is available from OCA.

Reference

The high resolution crystal structure of DMSO reductase in complex with DMSO., McAlpine AS, McEwan AG, Bailey S, J Mol Biol. 1998 Jan 30;275(4):613-23. PMID:9466935

Page seeded by OCA on Thu Mar 20 19:09:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4dmr"
Personal tools