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4lyo
From Proteopedia
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| - | [[Image:4lyo.jpg|left|200px]] | + | [[Image:4lyo.jpg|left|200px]] |
| - | + | ||
| - | '''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER''' | + | {{Structure |
| + | |PDB= 4lyo |SIZE=350|CAPTION= <scene name='initialview01'>4lyo</scene>, resolution 2.05Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4LYO is a [ | + | 4LYO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LYO OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:[http:// | + | X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9659395 9659395] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
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[[Category: lysozyme]] | [[Category: lysozyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:10:13 2008'' |
Revision as of 17:10, 20 March 2008
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| , resolution 2.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CROSS-LINKED CHICKEN LYSOZYME CRYSTAL IN NEAT ACETONITRILE, THEN BACK-SOAKED IN WATER
Overview
Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.
About this Structure
4LYO is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture., Wang Z, Zhu G, Huang Q, Qian M, Shao M, Jia Y, Tang Y, Biochim Biophys Acta. 1998 May 19;1384(2):335-44. PMID:9659395
Page seeded by OCA on Thu Mar 20 19:10:13 2008
Categories: Gallus gallus | Lysozyme | Single protein | Huang, Q. | Jia, Y. | Qian, M. | Shao, M. | Tang, Y. | Wang, Z. | Zhu, G. | Cross-linked | Hydrolase | Hydrolase (o-glycosyl)
