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3zpx

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{{STRUCTURE_3zpx| PDB=3zpx | SCENE= }}
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==USTILAGO MAYDIS LIPASE UM03410, SHORT FORM WITHOUT FLAP==
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===USTILAGO MAYDIS LIPASE UM03410, SHORT FORM WITHOUT FLAP===
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<StructureSection load='3zpx' size='340' side='right' caption='[[3zpx]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
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{{ABSTRACT_PUBMED_22294433}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3zpx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corn_smut_fungus Corn smut fungus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZPX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zpx RCSB], [http://www.ebi.ac.uk/pdbsum/3zpx PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Ustilago maydis lipase UM03410 belongs to the mostly unexplored Candida antarctica lipase (CAL-A) subfamily. The two lipases with [corrected] the highest identity are a lipase from Sporisorium reilianum and the prototypic CAL-A. In contrast to the other CAL-A-type lipases, this hypothetical U. maydis lipase is annotated to possess a prolonged N-terminus of unknown function. Here, we show for the first time the recombinant expression of two versions of lipase UM03410: the full-length form (lipUMf) and an Nterminally truncated form (lipUMs). For comparison to the prototype, the expression of recombinant CAL-A in E. coli was investigated. Although both forms of lipase UM03410 could be expressed functionally in E. coli, the N-terminally truncated form (lipUMs) demonstrated significantly higher activities towards p-nitrophenyl esters. The functional expression of the N-terminally truncated lipase was further optimized by the appropriate choice of the E. coli strain, lowering the cultivation temperature to 20 degrees C and enrichment of the cultivation medium with glucose. Primary characteristics of the recombinant lipase are its pH optimum in the range of 6.5-7.0 and its temperature optimum at 55 degrees C. As is typical for lipases, lipUM03410 shows preference for long chain fatty acid esters with myristic acid ester (C14:0 ester) being the most preferred one.More importantly, lipUMs exhibits an inherent preference for C18:1Delta9 trans and C18:1Delta11 trans-fatty acid esters similar to CAL-A. Therefore, the short form of this U. maydis lipase is the only other currently known lipase with a distinct trans-fatty acid selectivity.
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==About this Structure==
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The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity.,Brundiek H, Sass S, Evitt A, Kourist R, Bornscheuer UT Appl Microbiol Biotechnol. 2012 Apr;94(1):141-50. doi: 10.1007/s00253-012-3903-9., Epub 2012 Feb 1. PMID:22294433<ref>PMID:22294433</ref>
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[[3zpx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPX OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:022294433</ref><references group="xtra"/><references/>
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</div>
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==See Also==
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*[[Lipase|Lipase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Corn smut fungus]]
[[Category: Triacylglycerol lipase]]
[[Category: Triacylglycerol lipase]]
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[[Category: Hinrichs, W.]]
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[[Category: Hinrichs, W]]
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[[Category: Palm, G J.]]
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[[Category: Palm, G J]]
[[Category: Alpha beta hydrolase]]
[[Category: Alpha beta hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 09:17, 4 January 2015

USTILAGO MAYDIS LIPASE UM03410, SHORT FORM WITHOUT FLAP

3zpx, resolution 1.99Å

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