4led
From Proteopedia
(Difference between revisions)
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| - | + | ==The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms== | |
| - | + | <StructureSection load='4led' size='340' side='right' caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4led]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_str._c_1433 Pseudomonas aeruginosa str. c 1433]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LED FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4le7|4le7]], [[4lea|4lea]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [http://www.ebi.ac.uk/pdbsum/4led PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Lectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins. | ||
| - | + | Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor.,McCaughey LC, Grinter R, Josts I, Roszak AW, Waloen KI, Cogdell RJ, Milner J, Evans T, Kelly S, Tucker NP, Byron O, Smith B, Walker D PLoS Pathog. 2014 Feb 6;10(2):e1003898. doi: 10.1371/journal.ppat.1003898., eCollection 2014 Feb. PMID:24516380<ref>PMID:24516380</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Cogdell, C J | + | == References == |
| - | [[Category: Grinter, R | + | <references/> |
| - | [[Category: Mccaughey, L | + | __TOC__ |
| - | [[Category: Roszak, A W | + | </StructureSection> |
| - | [[Category: Walker, D | + | [[Category: Pseudomonas aeruginosa str. c 1433]] |
| + | [[Category: Cogdell, C J]] | ||
| + | [[Category: Grinter, R]] | ||
| + | [[Category: Mccaughey, L]] | ||
| + | [[Category: Roszak, A W]] | ||
| + | [[Category: Walker, D]] | ||
[[Category: Antimicrobial protein]] | [[Category: Antimicrobial protein]] | ||
[[Category: Bacteriocin]] | [[Category: Bacteriocin]] | ||
Revision as of 09:51, 5 January 2015
The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms
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