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4kdp
From Proteopedia
(Difference between revisions)
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| - | + | ==TcaR-ssDNA complex crystal structure reveals the novel ssDNA binding mechanism of the MarR family proteins== | |
| - | + | <StructureSection load='4kdp' size='340' side='right' caption='[[4kdp]], [[Resolution|resolution]] 3.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4kdp]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Staes Staes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KDP OCA]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SE_1937 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176280 STAES])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kdp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kdp RCSB], [http://www.ebi.ac.uk/pdbsum/4kdp PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The teicoplanin-associated locus regulator (TcaR) regulates gene expression of proteins on the intercellular adhesion (ica) locus involved in staphylococci poly-N-acetylglucosamine biosynthesis. The absence of TcaR increases poly-N-acetylglucosamine production and promotes biofilm formation. Until recently, the mechanism of multiple antibiotic resistance regulator family protein members, such as TcaR, was restricted to binding double-stranded DNA. However, we recently found that TcaR strongly interacts with single-stranded DNA, which is a new role for this family of proteins. In this study, we report Staphylococcus epidermidis TcaR-single-stranded DNA complex structures. Our model suggests that TcaR and single-stranded DNA form a 61-symmetry polymer composed of TcaR dimers with single-stranded DNA that wraps outside the polymer and 12 nt per TcaR dimer. Single-stranded DNA binding to TcaR involves a large conformational change at the DNA binding lobe. Several point mutations involving the single-stranded DNA binding surface validate interactions between single-stranded DNA and TcaR. Our results extend the novel role of multiple antibiotic resistance regulator family proteins in staphylococci. | ||
| - | + | TcaR-ssDNA complex crystal structure reveals new DNA binding mechanism of the MarR family proteins.,Chang YM, Ho CH, Chen CK, Maestre-Reyna M, Chang-Chien MW, Wang AH Nucleic Acids Res. 2014 Feb 14. PMID:24531929<ref>PMID:24531929</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Staes]] | ||
[[Category: Chang, Y M.]] | [[Category: Chang, Y M.]] | ||
[[Category: Chen, C K.M.]] | [[Category: Chen, C K.M.]] | ||
Revision as of 06:59, 14 May 2014
TcaR-ssDNA complex crystal structure reveals the novel ssDNA binding mechanism of the MarR family proteins
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