We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
4k0f
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal structure of lanosterol 14-alpha demethylase with intact transmembrane domain bound to itraconazole== | |
| - | + | <StructureSection load='4k0f' size='340' side='right' caption='[[4k0f]], [[Resolution|resolution]] 2.19Å' scene=''> | |
| - | {{ | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[4k0f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K0F FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1YN:2-[(2R)-BUTAN-2-YL]-4-{4-[4-(4-{[(2R,4S)-2-(2,4-DICHLOROPHENYL)-2-(1H-1,2,4-TRIAZOL-1-YLMETHYL)-1,3-DIOXOLAN-4-YL]METHOXY}PHENYL)PIPERAZIN-1-YL]PHENYL}-2,4-DIHYDRO-3H-1,2,4-TRIAZOL-3-ONE'>1YN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERG11, SCY_2394 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=307796 Baker's yeast])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k0f RCSB], [http://www.ebi.ac.uk/pdbsum/4k0f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bitopic integral membrane proteins with a single transmembrane helix play diverse roles in catalysis, cell signaling, and morphogenesis. Complete monospanning protein structures are needed to show how interaction between the transmembrane helix and catalytic domain might influence association with the membrane and function. We report crystal structures of full-length Saccharomyces cerevisiae lanosterol 14alpha-demethylase, a membrane monospanning cytochrome P450 of the CYP51 family that catalyzes the first postcyclization step in ergosterol biosynthesis and is inhibited by triazole drugs. The structures reveal a well-ordered N-terminal amphipathic helix preceding a putative transmembrane helix that would constrain the catalytic domain orientation to lie partly in the lipid bilayer. The structures locate the substrate lanosterol, identify putative substrate and product channels, and reveal constrained interactions with triazole antifungal drugs that are important for drug design and understanding drug resistance. | ||
| - | + | Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer.,Monk BC, Tomasiak TM, Keniya MV, Huschmann FU, Tyndall JD, O'Connell JD 3rd, Cannon RD, McDonald JG, Rodriguez A, Finer-Moore JS, Stroud RM Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3865-70. doi:, 10.1073/pnas.1324245111. Epub 2014 Feb 3. PMID:24613931<ref>PMID:24613931</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Cannon, R D | + | == References == |
| - | [[Category: Finer-Morre, J | + | <references/> |
| - | [[Category: Huschmann, F U | + | __TOC__ |
| - | [[Category: III, J D.O Connell | + | </StructureSection> |
| - | [[Category: Keniya, M V | + | [[Category: Baker's yeast]] |
| - | [[Category: Monk, B C | + | [[Category: Cannon, R D]] |
| - | [[Category: Stroud, R M | + | [[Category: Finer-Morre, J]] |
| - | [[Category: Tomasiak, T M | + | [[Category: Huschmann, F U]] |
| - | [[Category: Tyndall, J D.A | + | [[Category: III, J D.O Connell]] |
| + | [[Category: Keniya, M V]] | ||
| + | [[Category: Monk, B C]] | ||
| + | [[Category: Stroud, R M]] | ||
| + | [[Category: Tomasiak, T M]] | ||
| + | [[Category: Tyndall, J D.A]] | ||
[[Category: Demethylation]] | [[Category: Demethylation]] | ||
[[Category: Lanosterol]] | [[Category: Lanosterol]] | ||
Revision as of 12:11, 4 January 2015
Crystal structure of lanosterol 14-alpha demethylase with intact transmembrane domain bound to itraconazole
| |||||||||||
