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4fdx
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| - | + | ==Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum== | |
| - | + | <StructureSection load='4fdx' size='340' side='right' caption='[[4fdx]], [[Resolution|resolution]] 1.64Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4fdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metpp Metpp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FDX FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4faz|4faz]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mpe_A3323 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420662 METPP])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxaloacetate_tautomerase Oxaloacetate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.2 5.3.2.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fdx RCSB], [http://www.ebi.ac.uk/pdbsum/4fdx PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order to understand the roles of the pathways, the 4-oxalocrotonate tautomerase (4-OT) isozyme from each pathway was characterized. Tautomerase I and tautomerase II have the lowest pairwise sequence identity (35%) among the isozyme pairs in the parallel pathways, and could offer insight into substrate preferences and pathway functions. The kinetic parameters of tautomerase I and tautomerase II were determined using 2-hydroxymuconate and 5-(methyl)-2-hydroxymuconate. Both tautomerase I and tautomerase II process the substrates, but with different efficiencies. Crystal structures were determined for both tautomerase I and tautomerase II, at 1.57 and 1.64A resolution, respectively. The backbones of tautomerase I and tautomerase II are highly similar, but have distinct active site environments. The results, in combination with those for other structurally and kinetically characterized 4-OT isozymes, suggest that tautomerase I catalyzes the tautomerization of both 2-hydroxymuconate and alkyl derivatives, whereas tautomerase II might specialize in other aromatic hydrocarbon metabolites. | ||
| - | + | Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.,Terrell CR, Burks EA, Whitman CP, Hoffman DW Arch Biochem Biophys. 2013 Sep 1;537(1):113-24. doi: 10.1016/j.abb.2013.06.016., Epub 2013 Jul 4. PMID:23831510<ref>PMID:23831510</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Metpp]] | [[Category: Metpp]] | ||
[[Category: Oxaloacetate tautomerase]] | [[Category: Oxaloacetate tautomerase]] | ||
| - | [[Category: Hoffman, D W | + | [[Category: Hoffman, D W]] |
| - | [[Category: Terrell, C R | + | [[Category: Terrell, C R]] |
| - | [[Category: Whitman, C P | + | [[Category: Whitman, C P]] |
| - | + | ||
[[Category: Alpha/beta barrel]] | [[Category: Alpha/beta barrel]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Tautomerase superfamily]] | [[Category: Tautomerase superfamily]] | ||
Revision as of 11:33, 20 January 2015
Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum
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