4p5j
From Proteopedia
(Difference between revisions)
m (Protected "4p5j" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | ''' | + | ==Crystal structure of the tRNA-like structure from Turnip Yellow Mosaic Virus (TYMV), a tRNA mimicking RNA== |
| + | <StructureSection load='4p5j' size='340' side='right' caption='[[4p5j]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4p5j]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P5J FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IRI:IRIDIUM+HEXAMMINE+ION'>IRI</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p5j RCSB], [http://www.ebi.ac.uk/pdbsum/4p5j PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Turnip yellow mosaic virus (TYMV) contains a tRNA-like structure (TLS) in its 3' untranslated region (3' UTR). This highly structured element induces valylation of the viral RNA by host cell enzymes and is important for virus proliferation. Directly upstream of the TYMV TLS is an upstream pseudoknot domain (UPD) that has been considered to be structurally distinct from the TLS. However, using a combination of functional, biochemical, and biophysical assays, we show that the entire 3' UTR of the viral genome is a single structured element in the absence of cellular protein. This packing architecture stabilizes the RNA structure and creates a better substrate for aminoacylation, and thus the UPD and TLS are functionally and structurally coupled. It has been proposed that the TYMV TLS acts as a molecular switch between translation and replication. Our results suggest that this putative switch could be based on structural changes within the global architecture of the UTR induced by interactions with the ribosome. The TYMV TLS.UPD might demonstrate how RNA structural plasticity can play a role in regulation of biological processes. | ||
| - | + | Multi-domain packing in the aminoacylatable 3' end of a plant viral RNA.,Hammond JA, Rambo RP, Kieft JS J Mol Biol. 2010 Jun 11;399(3):450-63. doi: 10.1016/j.jmb.2010.04.016. Epub 2010 , Apr 14. PMID:20398674<ref>PMID:20398674</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Colussi, T M.]] | ||
| + | [[Category: Costantino, D A.]] | ||
| + | [[Category: Hammond, J A.]] | ||
| + | [[Category: Kieft, J S.]] | ||
| + | [[Category: Nix, J C.]] | ||
| + | [[Category: Ruehle, G M.]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Trna-mimic viral rna pseudoknot multifunctional]] | ||
Revision as of 05:07, 4 June 2014
Crystal structure of the tRNA-like structure from Turnip Yellow Mosaic Virus (TYMV), a tRNA mimicking RNA
| |||||||||||
