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7rsa
From Proteopedia
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| - | [[Image:7rsa.gif|left|200px]] | + | [[Image:7rsa.gif|left|200px]] |
| - | + | ||
| - | '''STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS''' | + | {{Structure |
| + | |PDB= 7rsa |SIZE=350|CAPTION= <scene name='initialview01'>7rsa</scene>, resolution 1.26Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 7RSA is a [ | + | 7RSA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RSA OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:[http:// | + | Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3401445 3401445] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: hydrolase (phosphoric diester)]] | [[Category: hydrolase (phosphoric diester)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:15:03 2008'' |
Revision as of 17:15, 20 March 2008
| |||||||
| , resolution 1.26Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS
Overview
The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme.
About this Structure
7RSA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:3401445
Page seeded by OCA on Thu Mar 20 19:15:03 2008
