4bff
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus in the reduced form== | |
| - | + | <StructureSection load='4bff' size='340' side='right' caption='[[4bff]], [[Resolution|resolution]] 2.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4bff]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BFF FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bfj|4bfj]], [[4bfk|4bfk]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Superoxide_reductase Superoxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.2 1.15.1.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bff OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bff RCSB], [http://www.ebi.ac.uk/pdbsum/4bff PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU]] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). | [[http://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU]] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Neelaredoxins are a type of superoxide reductase (SOR), which are blue 14 kDa metalloproteins with a catalytic nonhaem iron centre coordinated by four histidines and one cysteine in the ferrous form. Anaerobic organisms such as Archaeoglobus fulgidus, a hyperthermophilic sulfate-reducing archaeon, have developed defence mechanisms against toxic oxygen species in which superoxide reductases play a key role. SOR is responsible for scavenging toxic superoxide anion radicals (O(2)(*-)), catalysing the one-electron reduction of superoxide to hydrogen peroxide. Crystals of recombinant A. fulgidus neelaredoxin in the oxidized form (13.7 kDa, 125 residues) were obtained using polyethylene glycol and ammonium sulfate. These crystals diffracted to 1.9 A resolution and belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 75.72, c = 185.44 A. Cell-content analysis indicated the presence of a tetramer in the asymmetric unit, with a Matthews coefficient (V(M)) of 2.36 A(3) Da(-1) and an estimated solvent content of 48%. The three-dimensional structure was determined by the MAD method and is currently under refinement. | ||
| + | |||
| + | Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin.,Bandeiras TM, Romao CV, Rodrigues JV, Teixeira M, Matias PM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):316-9. doi:, 10.1107/S1744309110000916. Epub 2010 Feb 24. PMID:20208170<ref>PMID:20208170</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Superoxide Reductase|Superoxide Reductase]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arcfl]] | ||
[[Category: Superoxide reductase]] | [[Category: Superoxide reductase]] | ||
| - | [[Category: Bandeiras, T M | + | [[Category: Bandeiras, T M]] |
| - | [[Category: Barradas, A R | + | [[Category: Barradas, A R]] |
| - | [[Category: Matias, P M | + | [[Category: Matias, P M]] |
| - | [[Category: Pinho, F G | + | [[Category: Pinho, F G]] |
| - | [[Category: Pinto, A F | + | [[Category: Pinto, A F]] |
| - | [[Category: Rodrigues, J V | + | [[Category: Rodrigues, J V]] |
| - | [[Category: Romao, C V | + | [[Category: Romao, C V]] |
| - | [[Category: Sousa, C M | + | [[Category: Sousa, C M]] |
| - | [[Category: Teixeira, M | + | [[Category: Teixeira, M]] |
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Oxygen detoxification]] | [[Category: Oxygen detoxification]] | ||
Revision as of 18:42, 25 December 2014
Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus in the reduced form
| |||||||||||
