This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3mb8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3mb8| PDB=3mb8 | SCENE= }}
+
==Crystal structure of purine nucleoside phosphorylase from toxoplasma gondii in complex with immucillin-H==
-
===Crystal structure of purine nucleoside phosphorylase from toxoplasma gondii in complex with immucillin-H===
+
<StructureSection load='3mb8' size='340' side='right' caption='[[3mb8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24585883}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3mb8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MB8 OCA]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMH:1,4-DIDEOXY-4-AZA-1-(S)-(9-DEAZAHYPOXANTHIN-9-YL)-D-RIBITOL'>IMH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PNP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
 +
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mb8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mb8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mb8 RCSB], [http://www.ebi.ac.uk/pdbsum/3mb8 PDBsum]</span></td></tr>
 +
<table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The intracellular pathogen Toxoplasma gondii is a purine auxotroph that relies on purine salvage for proliferation. We have optimized T. gondii purine nucleoside phosphorylase (TgPNP) stability and crystallized TgPNP with phosphate and Immucillin-H, a transition-state analogue that has high affinity for the enzyme. Immucillin-H bound to TgPNP with a dissociation constant of 370 pM, the highest affinity of eleven immucillins selected to probe the catalytic site. The specificity for transition-state analogues indicated an early dissociative transition state for TgPNP. Compared with PfPNP, large substituents surrounding the 5' -hydroxyl group of inhibitors demonstrate reduced capacity for TgPNP inhibition. Catalytic discrimination against large 5' groups is consistent with the inability of TgPNP to catalyze the phosphorylysis of 5' -methylthioinosine to hypoxanthine. In contrast to mammalian PNP, the 2' -hydroxyl group is crucial for inhibitor binding in the catalytic site of TgPNP. This first crystal structure of TgPNP describes the basis for discrimination against 5' -methylthioinosine and similarly 5' -hydroxy-substituted immucillins; structural differences reflect the unique adaptations of purine salvage pathways of Apicomplexa.
-
==About this Structure==
+
INHIBITION AND STRUCTURE OF TOXOPLASMA GONDII PURINE NUCLEOSIDE PHOSPHORYLASE.,Donaldson TM, Cassera MB, Ho MC, Zhan C, Merino EF, Evans GB, Tyler PC, Almo SC, Schramm VL, Kim K Eukaryot Cell. 2014 Feb 28. PMID:24585883<ref>PMID:24585883</ref>
-
[[3mb8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MB8 OCA].
+
-
==See Also==
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
*[[Purine nucleoside phosphorylase|Purine nucleoside phosphorylase]]
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:024585883</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: Purine-nucleoside phosphorylase]]
[[Category: Purine-nucleoside phosphorylase]]
[[Category: Toxgo]]
[[Category: Toxgo]]

Revision as of 06:58, 14 May 2014

Crystal structure of purine nucleoside phosphorylase from toxoplasma gondii in complex with immucillin-H

3mb8, resolution 1.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3mb8"
Views
Personal tools
Navigation
Toolbox