4o88
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal structure of a C-tagged Nuclease== |
| + | <StructureSection load='4o88' size='340' side='right' caption='[[4o88]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4o88]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O88 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o87|4o87]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o88 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4o88 RCSB], [http://www.ebi.ac.uk/pdbsum/4o88 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT incises a synthetic tRNA(Gln(UUG)) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems. | ||
| - | + | Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination.,Chakravarty AK, Smith P, Jalan R, Shuman S Cell Rep. 2014 Apr 24;7(2):339-47. doi: 10.1016/j.celrep.2014.03.034. Epub 2014, Apr 13. PMID:24726365<ref>PMID:24726365</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chakravarty, A K.]] | ||
| + | [[Category: Shuman, S.]] | ||
| + | [[Category: Smith, P.]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Novel fold]] | ||
| + | [[Category: Nuclease]] | ||
Revision as of 10:36, 20 October 2014
Crystal structure of a C-tagged Nuclease
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