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4oxp
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==X-ray crystal structure of the S1 and 5'-sensor domains of RNase E from Caulobacter crescentus== |
| + | <StructureSection load='4oxp' size='340' side='right' caption='[[4oxp]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4oxp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OXP FirstGlance]. <br> | ||
| + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_E Ribonuclease E], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.12 3.1.26.12] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4oxp RCSB], [http://www.ebi.ac.uk/pdbsum/4oxp PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central component of the RNA degradosome assembly. A similar RNA degradosome assembly has been described in the alpha-proteobacterium Caulobacter crescentus, with the interacting partners of RNase E identified as the Kreb's cycle enzyme aconitase, a DEAD-box RNA helicase RhlB and the exoribonuclease polynucleotide phosphorylase. Here we report that an additional degradosome component is the essential exoribonuclease RNase D, and its recognition site within RNase E is identified. We show that, unlike its E. coli counterpart, C. crescentus RhlB interacts directly with a segment of the N-terminal catalytic domain of RNase E. The crystal structure of a portion of C. crescentus RNase E encompassing the helicase-binding region is reported. This structure reveals that an inserted segment in the S1 domain adopts an alpha-helical conformation, despite being predicted to be natively unstructured. We discuss the implications of these findings for the organization and mechanisms of the RNA degradosome. | ||
| - | + | Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome.,Voss JE, Luisi BF, Hardwick SW Nucleic Acids Res. 2014 Dec 1;42(21):13294-305. doi: 10.1093/nar/gku1134. Epub, 2014 Nov 11. PMID:25389270<ref>PMID:25389270</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ribonuclease E]] | ||
| + | [[Category: Hardwick, S W]] | ||
| + | [[Category: Luisi, B F.L]] | ||
| + | [[Category: Voss, J E]] | ||
| + | [[Category: Endoribonuclease]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Rnase e]] | ||
Revision as of 09:27, 3 December 2014
X-ray crystal structure of the S1 and 5'-sensor domains of RNase E from Caulobacter crescentus
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