4bgl
From Proteopedia
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| - | + | ==Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus== | |
| - | + | <StructureSection load='4bgl' size='340' side='right' caption='[[4bgl]], [[Resolution|resolution]] 1.90Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4bgl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BGL FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bgl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bgl RCSB], [http://www.ebi.ac.uk/pdbsum/4bgl PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU]] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). | [[http://www.uniprot.org/uniprot/SOR_ARCFU SOR_ARCFU]] Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Neelaredoxins are a type of superoxide reductase (SOR), which are blue 14 kDa metalloproteins with a catalytic nonhaem iron centre coordinated by four histidines and one cysteine in the ferrous form. Anaerobic organisms such as Archaeoglobus fulgidus, a hyperthermophilic sulfate-reducing archaeon, have developed defence mechanisms against toxic oxygen species in which superoxide reductases play a key role. SOR is responsible for scavenging toxic superoxide anion radicals (O(2)(*-)), catalysing the one-electron reduction of superoxide to hydrogen peroxide. Crystals of recombinant A. fulgidus neelaredoxin in the oxidized form (13.7 kDa, 125 residues) were obtained using polyethylene glycol and ammonium sulfate. These crystals diffracted to 1.9 A resolution and belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 75.72, c = 185.44 A. Cell-content analysis indicated the presence of a tetramer in the asymmetric unit, with a Matthews coefficient (V(M)) of 2.36 A(3) Da(-1) and an estimated solvent content of 48%. The three-dimensional structure was determined by the MAD method and is currently under refinement. | ||
| + | |||
| + | Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin.,Bandeiras TM, Romao CV, Rodrigues JV, Teixeira M, Matias PM Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):316-9. doi:, 10.1107/S1744309110000916. Epub 2010 Feb 24. PMID:20208170<ref>PMID:20208170</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Superoxide Reductase|Superoxide Reductase]] | |
| - | [[Category: Bandeiras, T M | + | == References == |
| - | [[Category: Barradas, A R | + | <references/> |
| - | [[Category: Matias, P M | + | __TOC__ |
| - | [[Category: Pinho, F G | + | </StructureSection> |
| - | [[Category: Pinto, A F | + | [[Category: Arcfl]] |
| - | [[Category: Rodrigues, J V | + | [[Category: Bandeiras, T M]] |
| - | [[Category: Romao, C V | + | [[Category: Barradas, A R]] |
| - | [[Category: Sousa, C M | + | [[Category: Matias, P M]] |
| - | [[Category: Teixeira, M | + | [[Category: Pinho, F G]] |
| + | [[Category: Pinto, A F]] | ||
| + | [[Category: Rodrigues, J V]] | ||
| + | [[Category: Romao, C V]] | ||
| + | [[Category: Sousa, C M]] | ||
| + | [[Category: Teixeira, M]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Oxygen detoxification]] | [[Category: Oxygen detoxification]] | ||
Revision as of 08:21, 25 December 2014
Superoxide reductase (Neelaredoxin) from Archaeoglobus fulgidus
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