3lcm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3lcm| PDB=3lcm | SCENE= }}
+
==Crystal structure of Smu.1420 from Streptococcus mutans UA159==
-
===Crystal structure of Smu.1420 from Streptococcus mutans UA159===
+
<StructureSection load='3lcm' size='340' side='right' caption='[[3lcm]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24699637}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3lcm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strmu Strmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LCM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LCM FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">smu.1420 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210007 STRMU])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NAD(P)H_dehydrogenase_(quinone) NAD(P)H dehydrogenase (quinone)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.2 1.6.5.2] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lcm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lcm RCSB], [http://www.ebi.ac.uk/pdbsum/3lcm PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The smu.1420 gene from the cariogenic pathogen Streptococcus mutans encodes a putative protein which has sequence homology to NQO [ NAD(P)H: quinone oxidoreductase] family members, including mammalian NQO and bacterial MdaB (modulator of drug activity B). NQO can detoxify quinones by converting them to hydroquinones and prevent the generation of reactive oxygen species. Thus, comprehensive studies on Smu.1420 will be important for uncovering the antioxidation and antidrug mechanisms of S. mutans. Here, the catalytic properties of Smu.1420 have been characterized, and its structure was determined in complexes with NADP(+) and menadione, respectively. Smu.1420 binds menadione directly and exhibits a pronounced preference for NADPH over NADH as a substrate, demonstrating that it is an NADPH-specific quinone oxidoreductase. The structure of Smu.1420 shows a compact homodimer with two substrate pockets located in the cleft of the dimer interface. The nicotinamide moiety of NADP(+) is bound on top of the isoalloxazine moiety of the FAD cofactor and overlaps with the binding site of menadione, suggesting a hydride-transfer process from NADPH to FAD and then to menadione. Two strongly basic patches near the substrate pocket are expected to confer the preference for NADPH over NADH. These studies shed light on future drug development against the cariogenic pathogen S. mutans.
-
==About this Structure==
+
Structural and biochemical characterization of MdaB from cariogenic Streptococcus mutans reveals an NADPH-specific quinone oxidoreductase.,Wang Z, Li L, Dong YH, Su XD Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):912-21. doi:, 10.1107/S1399004713033749. Epub 2014 Mar 19. PMID:24699637<ref>PMID:24699637</ref>
-
[[3lcm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strmu Strmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LCM OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<ref group="xtra">PMID:024699637</ref><references group="xtra"/><references/>
+
</div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Strmu]]
[[Category: Strmu]]
-
[[Category: Su, X D.]]
+
[[Category: Su, X D]]
-
[[Category: Wang, Z X.]]
+
[[Category: Wang, Z X]]
[[Category: Mdab]]
[[Category: Mdab]]
[[Category: Nadph:quinone oxidoreductase]]
[[Category: Nadph:quinone oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Smu 1420]]
[[Category: Smu 1420]]

Revision as of 10:19, 25 January 2015

Crystal structure of Smu.1420 from Streptococcus mutans UA159

3lcm, resolution 1.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3lcm"
Personal tools