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4jho
From Proteopedia
(Difference between revisions)
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| - | + | ==Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase== | |
| - | + | <StructureSection load='4jho' size='340' side='right' caption='[[4jho]], [[Resolution|resolution]] 2.21Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4jho]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Indian_rice Indian rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JHO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4jie|4jie]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-mannosidase Beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.25 3.2.1.25] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jho RCSB], [http://www.ebi.ac.uk/pdbsum/4jho PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rice Os7BGlu26 is a GH1 family glycoside hydrolase with a threefold higher kcat/Km value for 4-nitrophenyl beta-D-mannoside (4NPMan) compared with 4-nitrophenyl beta-D-glucoside (4NPGlc). To investigate its selectivity for beta-D-mannoside and beta-D-glucoside substrates, the structures of apo Os7BGlu26 at a resolution of 2.20 A and of Os7BGlu26 with mannose at a resolution of 2.45 A were elucidated from isomorphous crystals in space group P212121. The (beta/alpha)8-barrel structure is similar to other GH1 family structures, but with a narrower active-site cleft. The Os7BGlu26 structure with D-mannose corresponds to a product complex, with beta-D-mannose in the (1)S5 skew-boat conformation. Docking of the (1)S3, (1)S5, (2)SO and (3)S1 pyranose-ring conformations of 4NPMan and 4NPGlc substrates into the active site of Os7BGlu26 indicated that the lowest energies were in the (1)S5 and (1)S3 skew-boat conformations. Comparison of these docked conformers with other rice GH1 structures revealed differences in the residues interacting with the catalytic acid/base between enzymes with and without beta-D-mannosidase activity. The mutation of Tyr134 to Trp in Os7BGlu26 resulted in similar kcat/Km values for 4NPMan and 4NPGlc, while mutation of Tyr134 to Phe resulted in a 37-fold higher kcat/Km for 4NPMan than 4NPGlc. Mutation of Cys182 to Thr decreased both the activity and the selectivity for beta-D-mannoside. It was concluded that interactions with the catalytic acid/base play a significant role in glycon selection. | ||
| - | + | Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase.,Tankrathok A, Iglesias-Fernandez J, Luang S, Robinson RC, Kimura A, Rovira C, Hrmova M, Ketudat Cairns JR Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):2124-35. doi:, 10.1107/S0907444913020568. Epub 2013 Sep 20. PMID:24100330<ref>PMID:24100330</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Mannosidase|Mannosidase]] | *[[Mannosidase|Mannosidase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Beta-mannosidase]] | [[Category: Beta-mannosidase]] | ||
[[Category: Indian rice]] | [[Category: Indian rice]] | ||
| - | [[Category: Cairns, J R.Ketudat | + | [[Category: Cairns, J R.Ketudat]] |
| - | [[Category: Hrmova, M | + | [[Category: Hrmova, M]] |
| - | [[Category: Kimura, A | + | [[Category: Kimura, A]] |
| - | [[Category: Luang, S | + | [[Category: Luang, S]] |
| - | [[Category: Robinson, R C | + | [[Category: Robinson, R C]] |
| - | [[Category: Tankrathok, A | + | [[Category: Tankrathok, A]] |
[[Category: Beta-d-mannosidase]] | [[Category: Beta-d-mannosidase]] | ||
[[Category: Glycoside hydrolase]] | [[Category: Glycoside hydrolase]] | ||
Revision as of 14:31, 4 January 2015
Structural analysis and insights into glycon specificity of the rice GH1 Os7BGlu26 beta-D-mannosidase
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