1z2q
From Proteopedia
(Difference between revisions)
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| - | + | ==High-resolution solution structure of the LM5-1 FYVE domain from Leishmania major== | |
| - | + | <StructureSection load='1z2q' size='340' side='right' caption='[[1z2q]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1z2q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z2Q FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z2q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1z2q RCSB], [http://www.ebi.ac.uk/pdbsum/1z2q PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z2/1z2q_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | FYVE domain proteins play key roles in regulating membrane traffic in eukaryotic cells. The FYVE domain displays a remarkable specificity for the head group of the target lipid, phosphatidylinositol 3-phosphate (PtdIns[3]P). We have identified five putative FYVE domain proteins in the genome of the protozoan parasite Leishmania major, three of which are predicted to contain a functional PtdIns(3)P-binding site. The FYVE domain of one of these proteins, LmFYVE-1, bound PtdIns(3)P in liposome-binding assays and targeted GFP to acidified late endosomes/lysosomes in mammalian cells. The high-resolution solution structure of its N-terminal FYVE domain (LmFYVE-1[1-79]) was solved by nuclear magnetic resonance. Functionally significant clusters of residues of the LmFYVE-1 domain involved in PtdIns(3)P binding and dependence on low pH for tight binding were identified. This structure is the first trypanosomatid membrane trafficking protein to be determined and has been refined to high precision and accuracy using residual dipolar couplings. | ||
| - | + | A high-resolution solution structure of a trypanosomatid FYVE domain.,Mertens HD, Callaghan JM, Swarbrick JD, McConville MJ, Gooley PR Protein Sci. 2007 Nov;16(11):2552-9. Epub 2007 Sep 28. PMID:17905827<ref>PMID:17905827</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Leima]] | [[Category: Leima]] | ||
[[Category: Callaghan, J M.]] | [[Category: Callaghan, J M.]] | ||
Revision as of 11:40, 3 October 2014
High-resolution solution structure of the LM5-1 FYVE domain from Leishmania major
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