2vpr
From Proteopedia
(New page: 200px<br /><applet load="2vpr" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vpr, resolution 2.49Å" /> '''TET REPRESSOR CLASS ...) |
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| - | [[Image:2vpr.jpg|left|200px]] | + | [[Image:2vpr.jpg|left|200px]] |
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| - | '''TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG''' | + | {{Structure |
| + | |PDB= 2vpr |SIZE=350|CAPTION= <scene name='initialview01'>2vpr</scene>, resolution 2.49Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Binding+Site+For+Residue+Tdc+A+1206'>AC1</scene>, <scene name='pdbsite=AC2:Binding+Site+For+Residue+Mg+A+1207'>AC2</scene> and <scene name='pdbsite=AC3:Binding+Site+For+Residue+So4+A+1208'>AC3</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=TDC:'>TDC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2VPR is a [ | + | 2VPR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system., Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W, Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:[http:// | + | Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system., Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W, Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700280 10700280] |
[[Category: Pasteurella multocida]] | [[Category: Pasteurella multocida]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription regulator]] | [[Category: transcription regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:48:03 2008'' |
Revision as of 16:48, 20 March 2008
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| , resolution 2.49Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG
Overview
The tetracycline repressor (TetR) regulates the most abundant resistance mechanism against the antibiotic tetracycline in grain-negative bacteria. The TetR protein and its mutants are commonly used as control elements to regulate gene expression in higher eukaryotes. We present the crystal structure of the TetR homodimer in complex with its palindromic DNA operator at 2.5 A resolution. Comparison to the structure of TetR in complex with the inducer tetracycline-Mg2+ allows the mechanism of induction to be deduced. Inducer binding in the repressor core initiates conformational changes starting with C-terminal unwinding and shifting of the short helix a6 in each monomer. This forces a pendulum-like motion of helix a4, which increases the separation of the attached DNA binding domains by 3 A, abolishing the affinity of TetR for its operator DNA.
About this Structure
2VPR is a Single protein structure of sequence from Pasteurella multocida. Full crystallographic information is available from OCA.
Reference
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system., Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W, Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:10700280
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