2zcs

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(New page: 200px<br /><applet load="2zcs" size="350" color="white" frame="true" align="right" spinBox="true" caption="2zcs, resolution 2.03&Aring;" /> '''Crystal structure of...)
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[[Image:2zcs.jpg|left|200px]]<br /><applet load="2zcs" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2zcs.jpg|left|200px]]
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caption="2zcs, resolution 2.03&Aring;" />
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'''Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus complexed with bisphosphonate BPH-700'''<br />
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{{Structure
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|PDB= 2zcs |SIZE=350|CAPTION= <scene name='initialview01'>2zcs</scene>, resolution 2.03&Aring;
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|SITE= <scene name='pdbsite=AC1:B70+Binding+Site+For+Residue+A+640'>AC1</scene>
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|LIGAND= <scene name='pdbligand=B70:'>B70</scene>
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|ACTIVITY=
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|GENE= CrtM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])
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}}
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'''Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus complexed with bisphosphonate BPH-700'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ZCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=B70:'>B70</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:B70+Binding+Site+For+Residue+A+640'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCS OCA].
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2ZCS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZCS OCA].
==Reference==
==Reference==
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A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence., Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E, Science. 2008 Mar 7;319(5868):1391-4. Epub 2008 Feb 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18276850 18276850]
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A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence., Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E, Science. 2008 Mar 7;319(5868):1391-4. Epub 2008 Feb 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18276850 18276850]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:38:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:53 2008''

Revision as of 16:53, 20 March 2008

Image:2zcs.jpg


PDB ID 2zcs

Drag the structure with the mouse to rotate
, resolution 2.03Å
Sites:
Ligands:
Gene: CrtM (Staphylococcus aureus)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the C(30) carotenoid dehydrosqualene synthase from Staphylococcus aureus complexed with bisphosphonate BPH-700


Overview

Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. aureus posing a serious public health threat. The golden carotenoid pigment of S. aureus, staphyloxanthin, promotes resistance to reactive oxygen species and host neutrophil-based killing, and early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures of S. aureus dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural similarity to human squalene synthase (SQS). We screened nine SQS inhibitors and determined the structures of three, bound to CrtM. One, previously tested for cholesterol-lowering activity in humans, blocked staphyloxanthin biosynthesis in vitro (median inhibitory concentration approximately 100 nM), resulting in colorless bacteria with increased susceptibility to killing by human blood and to innate immune clearance in a mouse infection model. This finding represents proof of principle for a virulence factor-based therapy against S. aureus.

About this Structure

2ZCS is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence., Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E, Science. 2008 Mar 7;319(5868):1391-4. Epub 2008 Feb 14. PMID:18276850

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