2dry
From Proteopedia
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| - | [[Image:2dry.jpg|left|200px]] | + | [[Image:2dry.jpg|left|200px]] |
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| - | '''Crystal structure of the earthworm lectin C-terminal domain mutant''' | + | {{Structure |
| + | |PDB= 2dry |SIZE=350|CAPTION= <scene name='initialview01'>2dry</scene>, resolution 1.80Å | ||
| + | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+2001'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Residue+A+2002'>AC2</scene>, <scene name='pdbsite=AC3:So4+Binding+Site+For+Residue+A+2003'>AC3</scene>, <scene name='pdbsite=AC4:So4+Binding+Site+For+Residue+A+2004'>AC4</scene>, <scene name='pdbsite=AC5:Pge+Binding+Site+For+Residue+B+1001'>AC5</scene> and <scene name='pdbsite=AC6:Pge+Binding+Site+For+Residue+A+1002'>AC6</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=PGE:TRIETHYLENE GLYCOL'>PGE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the earthworm lectin C-terminal domain mutant''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2DRY is a [ | + | 2DRY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DRY OCA]. |
==Reference== | ==Reference== | ||
| - | Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http:// | + | Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683] |
[[Category: Lumbricus terrestris]] | [[Category: Lumbricus terrestris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:30:44 2008'' |
Revision as of 14:30, 20 March 2008
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| , resolution 1.80Å | |||||||
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| Sites: | , , , , and | ||||||
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the earthworm lectin C-terminal domain mutant
Overview
Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.
About this Structure
2DRY is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.
Reference
Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683
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