2ivf

Structural highlights

2ivf is a 3 chain structure with sequence from Aromatoleum aromaticum. Full crystallographic information is available from OCA.
Ligands: , , , , , , , , ,
Activity: Glucokinase, with EC number 2.7.1.2
Resources: FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Anaerobic degradation of hydrocarbons was discovered a decade ago, and ethylbenzene dehydrogenase was one of the first characterized enzymes involved. The structure of the soluble periplasmic 165 kDa enzyme was established at 1.88 A resolution. It is a heterotrimer. The alpha subunit contains the catalytic center with a molybdenum held by two molybdopterin-guanine dinucleotides, one with an open pyran ring, and an iron-sulfur cluster with a histidine ligand. During catalysis, electrons produced by substrate oxidation are transferred to a heme in the gamma subunit and then presumably to a separate cytochrome involved in nitrate respiration. The beta subunit contains four iron-sulfur clusters and is structurally related to ferredoxins. The gamma subunit is the first known protein with a methionine and a lysine as axial heme ligands. The catalytic product was modeled into the active center, showing the reaction geometry. A mechanism consistent with activity and inhibition data of ethylbenzene-related compounds is proposed.

Crystal structure of ethylbenzene dehydrogenase from Aromatoleum aromaticum.,Kloer DP, Hagel C, Heider J, Schulz GE Structure. 2006 Sep;14(9):1377-88. PMID:16962969^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

1. ↑ Kloer DP, Hagel C, Heider J, Schulz GE. Crystal structure of ethylbenzene dehydrogenase from Aromatoleum aromaticum. Structure. 2006 Sep;14(9):1377-88. PMID:16962969 doi:10.1016/j.str.2006.07.001