2vcf

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[[Image:2vcf.jpg|left|200px]]<br /><applet load="2vcf" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2vcf.jpg|left|200px]]
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caption="2vcf, resolution 1.80&Aring;" />
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'''STRUCTURE OF ISONIAZID (INH) BOUND TO CYTOSOLIC SOYBEAN ASCORBATE PEROXIDASE'''<br />
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{{Structure
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|PDB= 2vcf |SIZE=350|CAPTION= <scene name='initialview01'>2vcf</scene>, resolution 1.80&Aring;
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|SITE= <scene name='pdbsite=AC1:Isz+Binding+Site+For+Chain+X'>AC1</scene>, <scene name='pdbsite=AC2:Isz+Binding+Site+For+Chain+X'>AC2</scene>, <scene name='pdbsite=AC3:Hem+Binding+Site+For+Chain+X'>AC3</scene> and <scene name='pdbsite=AC4:Na+Binding+Site+For+Chain+X'>AC4</scene>
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ISZ:4-(DIAZENYLCARBONYL)PYRIDINE'>ISZ</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11]
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|GENE=
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}}
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'''STRUCTURE OF ISONIAZID (INH) BOUND TO CYTOSOLIC SOYBEAN ASCORBATE PEROXIDASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2VCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=ISZ:'>ISZ</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] Known structural/functional Sites: <scene name='pdbsite=AC1:Isz+Binding+Site+For+Chain+X'>AC1</scene>, <scene name='pdbsite=AC2:Isz+Binding+Site+For+Chain+X'>AC2</scene>, <scene name='pdbsite=AC3:Hem+Binding+Site+For+Chain+X'>AC3</scene> and <scene name='pdbsite=AC4:Na+Binding+Site+For+Chain+X'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VCF OCA].
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2VCF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VCF OCA].
==Reference==
==Reference==
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The Tuberculosis Prodrug Isoniazid Bound to Activating Peroxidases., Metcalfe C, Macdonald IK, Murphy EJ, Brown KA, Raven EL, Moody PC, J Biol Chem. 2008 Mar 7;283(10):6193-6200. Epub 2007 Dec 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18056997 18056997]
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The Tuberculosis Prodrug Isoniazid Bound to Activating Peroxidases., Metcalfe C, Macdonald IK, Murphy EJ, Brown KA, Raven EL, Moody PC, J Biol Chem. 2008 Mar 7;283(10):6193-6200. Epub 2007 Dec 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18056997 18056997]
[[Category: Glycine max]]
[[Category: Glycine max]]
[[Category: L-ascorbate peroxidase]]
[[Category: L-ascorbate peroxidase]]
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[[Category: peroxidase]]
[[Category: peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:43:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:45:30 2008''

Revision as of 16:45, 20 March 2008

Image:2vcf.jpg


PDB ID 2vcf

Drag the structure with the mouse to rotate
, resolution 1.80Å
Sites: , , and
Ligands: , and
Activity: L-ascorbate peroxidase, with EC number 1.11.1.11
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF ISONIAZID (INH) BOUND TO CYTOSOLIC SOYBEAN ASCORBATE PEROXIDASE


Overview

Isoniazid (INH, isonicotinic acid hydrazine) is one of only two therapeutic agents effective in treating tuberculosis. This prodrug is activated by the heme enzyme catalase peroxidase (KatG) endogenous to Mycobacterium tuberculosis but the mechanism of activation is poorly understood, in part because the binding interaction has not been properly established. The class I peroxidases ascorbate peroxidase (APX) and cytochrome c peroxidase (CcP) have active site structures very similar to KatG and are also capable of activating isoniazid. We report here the first crystal structures of complexes of isoniazid bound to APX and CcP. These are the first structures of isoniazid bound to any activating enzymes. The structures show that isoniazid binds close to the delta-heme edge in both APX and CcP, although the precise binding orientation varies slightly in the two cases. A second binding site for INH is found in APX at the gamma-heme edge close to the established ascorbate binding site, indicating that the gamma-heme edge can also support the binding of aromatic substrates. We also show that in an active site mutant of soybean APX (W41A) INH can bind directly to the heme iron to become an inhibitor and in a different mode when the distal histidine is replaced by alanine (H42A). These structures provide the first unambiguous evidence for the location of the isoniazid binding site in the class I peroxidases and provide rationalization of isoniazid resistance in naturally occurring KatG mutant strains of M. tuberculosis.

About this Structure

2VCF is a Single protein structure of sequence from Glycine max. Full crystallographic information is available from OCA.

Reference

The Tuberculosis Prodrug Isoniazid Bound to Activating Peroxidases., Metcalfe C, Macdonald IK, Murphy EJ, Brown KA, Raven EL, Moody PC, J Biol Chem. 2008 Mar 7;283(10):6193-6200. Epub 2007 Dec 5. PMID:18056997

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