1a6j
From Proteopedia
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|PDB= 1a6j |SIZE=350|CAPTION= <scene name='initialview01'>1a6j</scene>, resolution 2.35Å | |PDB= 1a6j |SIZE=350|CAPTION= <scene name='initialview01'>1a6j</scene>, resolution 2.35Å | ||
|SITE= <scene name='pdbsite=PH1:By+Comparison+w.+Iia+Enzymes,+This+Is+The+Phosphorylatio+...'>PH1</scene> and <scene name='pdbsite=PH2:By+Comparison+w.+Iia+Enzymes,+This+Is+The+Phosphorylatio+...'>PH2</scene> | |SITE= <scene name='pdbsite=PH1:By+Comparison+w.+Iia+Enzymes,+This+Is+The+Phosphorylatio+...'>PH1</scene> and <scene name='pdbsite=PH2:By+Comparison+w.+Iia+Enzymes,+This+Is+The+Phosphorylatio+...'>PH2</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a6j OCA], [http://www.ebi.ac.uk/pdbsum/1a6j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a6j RCSB]</span> | ||
}} | }} | ||
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[[Category: Reizer, J.]] | [[Category: Reizer, J.]] | ||
[[Category: Saier, M H.]] | [[Category: Saier, M H.]] | ||
| - | [[Category: BME]] | ||
| - | [[Category: SO4]] | ||
[[Category: nitrogen regulation]] | [[Category: nitrogen regulation]] | ||
[[Category: phosphotransferase system]] | [[Category: phosphotransferase system]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:34:23 2008'' |
Revision as of 15:34, 30 March 2008
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| , resolution 2.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , | ||||||
| Activity: | Protein-N(pi)-phosphohistidine--sugar phosphotransferase, with EC number 2.7.1.69 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NITROGEN REGULATORY BACTERIAL PROTEIN IIA-NITROGEN
Overview
The bacterial rpoN operon codes for sigma 54, which is the key sigma factor that, under nitrogen starvation conditions, activates the transcription of genes needed to assimilate ammonia and glutamate. The rpoN operon contains several other open reading frames that are cotranscribed with sigma 54. The product of one of these, the 17.9 kDa protein IIANtr, is homologous to IIA proteins of the phosphoenolpyruvate:sugar phosphotransferase (PTS) system. IIANtr influences the transcription of sigma 54-dependent genes through an unknown mechanism and may thereby provide a regulatory link between carbon and nitrogen metabolism. Here we describe the 2.35 A X-ray structure of Escherichia coli IIANtr. It is the first structure of a IIA enzyme from the fructose-mannitol family of the PTS. The enzyme displays a novel fold characterized by a central mixed parallel/anti-parallel beta-sheet surrounded by six alpha-helices. The active site His73 is situated in a shallow depression on the protein surface.
About this Structure
1A6J is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli., Bordo D, van Monfort RL, Pijning T, Kalk KH, Reizer J, Saier MH Jr, Dijkstra BW, J Mol Biol. 1998 May 29;279(1):245-55. PMID:9636714
Page seeded by OCA on Sun Mar 30 18:34:23 2008
