3sk0
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3sk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_mycoides_roseum"_grotenfelt_1889 "bacterium mycoides roseum" grotenfelt 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SK0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3sk0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_mycoides_roseum"_grotenfelt_1889 "bacterium mycoides roseum" grotenfelt 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SK0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SK0 FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>< | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bn6|1bn6]], [[1cqw|1cqw]]</td></tr> | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bn6|1bn6]], [[1cqw|1cqw]]</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dhaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1829 "Bacterium mycoides roseum" Grotenfelt 1889])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dhaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1829 "Bacterium mycoides roseum" Grotenfelt 1889])</td></tr> |
| - | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sk0 RCSB], [http://www.ebi.ac.uk/pdbsum/3sk0 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sk0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sk0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sk0 RCSB], [http://www.ebi.ac.uk/pdbsum/3sk0 PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/DHAA_RHORH DHAA_RHORH]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Expresses halogenase activity against 1-chloroalkanes of chain length C3 to C10, and also shows a very weak activity with 1,2-dichloroethane. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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Dynamics and hydration explain failed functional transformation in dehalogenase design.,Sykora J, Brezovsky J, Koudelakova T, Lahoda M, Fortova A, Chernovets T, Chaloupkova R, Stepankova V, Prokop Z, Smatanova IK, Hof M, Damborsky J Nat Chem Biol. 2014 Jun;10(6):428-30. doi: 10.1038/nchembio.1502. Epub 2014 Apr, 13. PMID:24727901<ref>PMID:24727901</ref> | Dynamics and hydration explain failed functional transformation in dehalogenase design.,Sykora J, Brezovsky J, Koudelakova T, Lahoda M, Fortova A, Chernovets T, Chaloupkova R, Stepankova V, Prokop Z, Smatanova IK, Hof M, Damborsky J Nat Chem Biol. 2014 Jun;10(6):428-30. doi: 10.1038/nchembio.1502. Epub 2014 Apr, 13. PMID:24727901<ref>PMID:24727901</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Dehalogenase|Dehalogenase]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Bacterium mycoides roseum grotenfelt 1889]] | [[Category: Bacterium mycoides roseum grotenfelt 1889]] | ||
[[Category: Haloalkane dehalogenase]] | [[Category: Haloalkane dehalogenase]] | ||
| - | [[Category: Damborsky, J | + | [[Category: Damborsky, J]] |
| - | [[Category: Koudelakova, T | + | [[Category: Koudelakova, T]] |
| - | [[Category: Kuta-Smatanova, I | + | [[Category: Kuta-Smatanova, I]] |
| - | [[Category: Lahoda, M | + | [[Category: Lahoda, M]] |
| - | [[Category: Mesters, J | + | [[Category: Mesters, J]] |
| - | [[Category: Stsiapanava, A | + | [[Category: Stsiapanava, A]] |
[[Category: Alpha/beta-hydrolase fold]] | [[Category: Alpha/beta-hydrolase fold]] | ||
[[Category: Catalytic pentad]] | [[Category: Catalytic pentad]] | ||
Revision as of 09:56, 25 December 2014
structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant DhaA12
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