4n0t
From Proteopedia
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n0t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n0t RCSB], [http://www.ebi.ac.uk/pdbsum/4n0t PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n0t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n0t RCSB], [http://www.ebi.ac.uk/pdbsum/4n0t PDBsum]</span></td></tr> | ||
<table> | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The spliceosome is a dynamic assembly of five small nuclear ribonucleoproteins (snRNPs) that removes introns from eukaryotic pre-mRNA. U6, the most conserved of the spliceosomal small nuclear RNAs (snRNAs), participates directly in catalysis. Here, we report the crystal structure of the Saccharomyces cerevisiae U6 snRNP core containing most of the U6 snRNA and all four RRM domains of the Prp24 protein. It reveals a unique interlocked RNP architecture that sequesters the 5' splice site-binding bases of U6 snRNA. RRMs 1, 2 and 4 of Prp24 form an electropositive groove that binds double-stranded RNA and may nucleate annealing of U4 and U6 snRNAs. Substitutions in Prp24 that suppress a mutation in U6 localize to direct RNA-protein contacts. Our results provide the most comprehensive view to date of a multi-RRM protein bound to RNA and reveal striking coevolution of protein and RNA structure. | ||
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| + | Core structure of the U6 small nuclear ribonucleoprotein at 1.7-A resolution.,Montemayor EJ, Curran EC, Liao HH, Andrews KL, Treba CN, Butcher SE, Brow DA Nat Struct Mol Biol. 2014 Jun;21(6):544-51. doi: 10.1038/nsmb.2832. Epub 2014 May, 18. PMID:24837192<ref>PMID:24837192</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:20, 2 July 2014
Core structure of the U6 small nuclear ribonucleoprotein at 1.7 Angstrom resolution
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