1a8z
From Proteopedia
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|PDB= 1a8z |SIZE=350|CAPTION= <scene name='initialview01'>1a8z</scene>, resolution 2.1Å | |PDB= 1a8z |SIZE=350|CAPTION= <scene name='initialview01'>1a8z</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CU1:COPPER (I) ION'>CU1</scene> | + | |LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a8z OCA], [http://www.ebi.ac.uk/pdbsum/1a8z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a8z RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Hasnain, S S.]] | [[Category: Hasnain, S S.]] | ||
[[Category: Ingledew, W J.]] | [[Category: Ingledew, W J.]] | ||
| - | [[Category: CU1]] | ||
[[Category: copper protein]] | [[Category: copper protein]] | ||
[[Category: direct method]] | [[Category: direct method]] | ||
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[[Category: sa]] | [[Category: sa]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:35:48 2008'' |
Revision as of 15:35, 30 March 2008
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| , resolution 2.1Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE DETERMINATION OF A 16.8KDA COPPER PROTEIN RUSTICYANIN AT 2.1A RESOLUTION USING ANOMALOUS SCATTERING DATA WITH DIRECT METHODS
Overview
The structure of rusticyanin, an acid-stable copper protein, has been determined at 2.1 A resolution by direct methods combined with the single-wavelength anomalous scattering (SAS) of copper (f" = 3.9 e-) and then conventionally refined (Rcryst = 18.7%, Rfree = 21.9%). This is the largest unknown protein structure (Mr approximately /= 16.8 kDa) to be determined using the SAS and direct-methods approach and demonstrates that by exploiting the anomalous signal at a single wavelength, direct methods can be used to determine phases at typical (approximately 2 A) macromolecular crystallographic resolutions. Extrapolating from the size of the anomalous signal for copper (f" approximately 4 e-), this result suggests that the approach could be used for proteins with molecular weights of up to 33 kDa per Se (f"max++ = 8 e- at the 'white line') and 80 kDa for a Pt derivative (f"max = 19 e- at the 'white line', L3 edge). The method provides a powerful alternative in solving a de novo protein structure without either preparing multiple crystals (i.e. isomorphous heavy-atom derivative plus native crystals) or collecting multi-wavelength anomalous diffraction (MAD) data.
About this Structure
1A8Z is a Single protein structure of sequence from Acidithiobacillus ferrooxidans. Full crystallographic information is available from OCA.
Reference
Structure determination of a 16.8 kDa copper protein at 2.1 A resolution using anomalous scattering data with direct methods., Harvey I, Hao Q, Duke EM, Ingledew WJ, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):629-35. PMID:9761859
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