1ana

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|PDB= 1ana |SIZE=350|CAPTION= <scene name='initialview01'>1ana</scene>, resolution 2.000&Aring;
|PDB= 1ana |SIZE=350|CAPTION= <scene name='initialview01'>1ana</scene>, resolution 2.000&Aring;
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|LIGAND= <scene name='pdbligand=C38:5-IODO-2&#39;-DEOXY-CYTIDINE-5&#39;-MONOPHOSPHATE'>C38</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ana OCA], [http://www.ebi.ac.uk/pdbsum/1ana PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ana RCSB]</span>
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[[Category: modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:59:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:43:48 2008''

Revision as of 15:43, 30 March 2008

Image:1ana.jpg


PDB ID 1ana

Drag the structure with the mouse to rotate
, resolution 2.000Å
Ligands: , ,
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G


Overview

The DNA oligomer of sequence IC-C-G-G has been synthesized, and its X-ray crystal structure solved at a resolution of 2.0 A, using anomalous scattering from iodines in phase analysis: 48 cycles of Jack-Levitt restrained least-squares refinement resulted in a residual error of 19.9% over all data, or 16.5% for two-sigma data. Two double-helical tetramers stack in the crystal to form a continuous octamer, except for the two missing phosphate connections across the center. The octamer has a mean helix rotation of 33.7 degrees (10.7 base-pairs per turn), rise of 2.87 A, mean inclination angle of base-pairs of 14 degrees, and mean base-pair propeller twist of +16.3 degrees. Local variations in both helix rotation and base plane roll angles, including those across the center of the octamer, are as predicted from base sequence by sum functions sigma 1 and sigma 2. The three known DNA octamers: IC-C-G-G/IC-C-G-G, G-G-T-A-T-A-C-C and G-G-C-C-G-G-C-C, make up a graded series in this order, with monotonically changing structural parameters. An exhaustive comparison of torsion angle correlations among the known A helices confirms some structural expectations and reveals some new features. 86 water molecules have been located per double-helical IC-C-G-G tetramer (the asymmetric unit), of which 451/2 per tetramer lie within a first hydrogen-bonded shell of hydration. No ordered water structure is observed comparable to the minor groove spine of hydration in B-DNA.

About this Structure

1ANA is a Protein complex structure of sequences from [1]. The following page contains interesting information on the relation of 1ANA with [DNA]. Full crystallographic information is available from OCA.

Reference

Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:6726797

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