1bo4
From Proteopedia
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|PDB= 1bo4 |SIZE=350|CAPTION= <scene name='initialview01'>1bo4</scene>, resolution 2.30Å | |PDB= 1bo4 |SIZE=350|CAPTION= <scene name='initialview01'>1bo4</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= AACC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=615 Serratia marcescens]) | |GENE= AACC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=615 Serratia marcescens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bo4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bo4 OCA], [http://www.ebi.ac.uk/pdbsum/1bo4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bo4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Vassilev, A.]] | [[Category: Vassilev, A.]] | ||
[[Category: Wolf, E.]] | [[Category: Wolf, E.]] | ||
| - | [[Category: COA]] | ||
| - | [[Category: SPD]] | ||
[[Category: aminoglycoside 3-n-acetyltransferase]] | [[Category: aminoglycoside 3-n-acetyltransferase]] | ||
[[Category: coa-binding]] | [[Category: coa-binding]] | ||
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[[Category: gcn5-related n-acetyltransferase]] | [[Category: gcn5-related n-acetyltransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:04:51 2008'' |
Revision as of 16:04, 30 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | AACC1 (Serratia marcescens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A GCN5-RELATED N-ACETYLTRANSFERASE: SERRATIA MARESCENS AMINOGLYCOSIDE 3-N-ACETYLTRANSFERASE
Overview
The X-ray structure of a canonical GCN5-related N-acetyltransferase (GNAT), Serratia marcescens aminoglycoside 3-N-acetyltransferase, bound to coenzyme A (CoA) has been determined at 2.3 A resolution. The single domain alpha/beta protein resembles a cupped right hand wrapped around a cylinder and consists of a highly curved, six-stranded beta sheet of mixed polarity that is sandwiched between four alpha helices. The structure includes all four conserved GNAT motifs (C, D, A, and B) and represents the catalytic core of this large enzyme superfamily. Acetyl CoA recognition is mediated by a betaalpha structure derived from GNAT motif A, which presents an invariant Arg/Gln-X-X-Gly-X-Gly/Ala segment for hydrogen bonding with the cofactor. Motif B contributes acidic residues to the binding site for the positively charged antibiotic substrate.
About this Structure
1BO4 is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase., Wolf E, Vassilev A, Makino Y, Sali A, Nakatani Y, Burley SK, Cell. 1998 Aug 21;94(4):439-49. PMID:9727487
Page seeded by OCA on Sun Mar 30 19:04:51 2008
