1bqk
From Proteopedia
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|PDB= 1bqk |SIZE=350|CAPTION= <scene name='initialview01'>1bqk</scene>, resolution 1.35Å | |PDB= 1bqk |SIZE=350|CAPTION= <scene name='initialview01'>1bqk</scene>, resolution 1.35Å | ||
|SITE= <scene name='pdbsite=COP:Oxidized+Cu+Coordination'>COP</scene> | |SITE= <scene name='pdbsite=COP:Oxidized+Cu+Coordination'>COP</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bqk OCA], [http://www.ebi.ac.uk/pdbsum/1bqk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bqk RCSB]</span> | ||
}} | }} | ||
| Line 32: | Line 35: | ||
[[Category: Shimomura, T.]] | [[Category: Shimomura, T.]] | ||
[[Category: Suzuki, S.]] | [[Category: Suzuki, S.]] | ||
| - | [[Category: CU]] | ||
[[Category: cuproprotein]] | [[Category: cuproprotein]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:06:15 2008'' |
Revision as of 16:06, 30 March 2008
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| , resolution 1.35Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
OXIDIZED PSEUDOAZURIN
Overview
The crystal structures of oxidized and reduced pseudoazurins from a denitrifying bacterium, Achromobacter cycloclastes IAM1013, have been determined at 1.35- and 1.6-A resolutions, respectively. The copper site in the oxidized state exhibits a distorted tetrahedral structure like those of other pseudoazurins. However, not only a small change of the copper geometry, but concerted peptide bond flips are identified. The imidazole ring of remote His6 has a hydrogen bonding distance of 2.73 A between N-delta1(His6) and O-gamma1(Thr36) in the oxidized protein. When the protein is reduced at pH 6.0, the imidazole ring rotates by 30.3 degrees and moves 1.00 A away from the position of the oxidized state. A new hydrogen bond between N-epsilon2(His6) and O-epsilon1(Glu4) is formed with a distance of 3.03 A, while the hydrogen bond between N-delta1(His6)-O-gamma1(Thr36) is maintained with an interatomic distance of 2.81 A. A concomitant peptide bond flip of main chain between Ile34 and Thr36 occurs.
About this Structure
1BQK is a Single protein structure of sequence from Achromobacter cycloclastes. Full crystallographic information is available from OCA.
Reference
Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine., Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y, J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:10364229
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