1brl
From Proteopedia
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|PDB= 1brl |SIZE=350|CAPTION= <scene name='initialview01'>1brl</scene>, resolution 2.4Å | |PDB= 1brl |SIZE=350|CAPTION= <scene name='initialview01'>1brl</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1brl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1brl OCA], [http://www.ebi.ac.uk/pdbsum/1brl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1brl RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Fisher, A J.]] | [[Category: Fisher, A J.]] | ||
[[Category: Rayment, I.]] | [[Category: Rayment, I.]] | ||
| - | [[Category: PO4]] | ||
[[Category: monooxygenase]] | [[Category: monooxygenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:06:46 2008'' |
Revision as of 16:06, 30 March 2008
| |||||||
| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION
Overview
Luciferases are a class of enzymes that generate light in the visible spectrum. Luciferase from luminous marine bacteria is an alpha-beta heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial luciferase from Vibrio harveyi has been determined to 2.4 A resolution. The structure was solved by a combination of multiple isomorphous replacement and molecular averaging between the two heterodimers in the asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and dimerization is mediated through a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the structurally similar subunits. The vicinity of the active site has been identified on the alpha subunit by correlations with similar protein motifs and previous biochemical studies. The structure presented here represents the first molecular model of a bioluminescent enzyme.
About this Structure
1BRL is a Protein complex structure of sequences from Vibrio harveyi. The following page contains interesting information on the relation of 1BRL with [Luciferase]. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:7756289
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