1bw6
From Proteopedia
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| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw6 OCA], [http://www.ebi.ac.uk/pdbsum/1bw6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bw6 RCSB]</span> | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:09:25 2008'' |
Revision as of 16:09, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1
Overview
CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
About this Structure
1BW6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:9451007
Page seeded by OCA on Sun Mar 30 19:09:25 2008
Categories: Homo sapiens | Single protein | Iwahara, J. | Kigawa, T. | Kitagawa, K. | Masumoto, H. | Okazaki, T. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Yokoyama, S. | Centromere protein | Dna-binding | Helix-turn-helix | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
