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3tlo

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tlo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified_human_coronavirus Unidentified human coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLO FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tlo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified_human_coronavirus Unidentified human coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TLO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TLO FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene><br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=694448 unidentified human coronavirus])</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=694448 unidentified human coronavirus])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tlo RCSB], [http://www.ebi.ac.uk/pdbsum/3tlo PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tlo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tlo RCSB], [http://www.ebi.ac.uk/pdbsum/3tlo PDBsum]</span></td></tr>
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<table>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/R1A_CVHNL R1A_CVHNL]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Unidentified human coronavirus]]
[[Category: Unidentified human coronavirus]]
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[[Category: Chuck, C P.]]
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[[Category: Chuck, C P]]
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[[Category: Wong, K B.]]
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[[Category: Wong, K B]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolysis]]
[[Category: Hydrolysis]]
[[Category: Protein binding]]
[[Category: Protein binding]]

Revision as of 03:52, 25 December 2014

Crystal structure of HCoV-NL63 3C-like protease

3tlo, resolution 1.60Å

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