1by9
From Proteopedia
| Line 6: | Line 6: | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= E2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= E2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=333760 Human papillomavirus type 16]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1by9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1by9 OCA], [http://www.ebi.ac.uk/pdbsum/1by9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1by9 RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1BY9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1BY9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BY9 OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of the E2 DNA-binding domain from human papillomavirus type 16: implications for its DNA binding-site selection mechanism., Hegde RS, Androphy EJ, J Mol Biol. 1998 Dec 18;284(5):1479-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878365 9878365] | Crystal structure of the E2 DNA-binding domain from human papillomavirus type 16: implications for its DNA binding-site selection mechanism., Hegde RS, Androphy EJ, J Mol Biol. 1998 Dec 18;284(5):1479-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9878365 9878365] | ||
| - | [[Category: Human papillomavirus type | + | [[Category: Human papillomavirus type 16]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Androphy, E J.]] | [[Category: Androphy, E J.]] | ||
| Line 28: | Line 31: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:10:43 2008'' |
Revision as of 16:10, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | E2 (Human papillomavirus type 16) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE E2 DNA-BINDING DOMAIN FROM HUMAN PAPILLOMAVIRUS TYPE-16: IMPLICATIONS FOR ITS DNA BINDING-SITE SELECTION MECHANISM
Overview
The crystal structure of the E2 DNA-binding domain from the high-risk cervical cancer-associated strain human papillomavirus type 16 (HPV-16) is described here. The papillomavirus E2 proteins regulate transcription from all viral promoters and are required for the initiation of replication in vivo. They belong to a family of viral proteins that form dimeric beta-barrels and use surface alpha-helices for DNA interaction. Although all E2 proteins recognize the same consensus, palindromic DNA sequence, proteins from different viral strains differ in their abilities to discriminate among their specific DNA-binding sites.The structure reported here reveals that while the overall fold of the HPV-16 E2 DNA-binding domain resembles that of its counterpart from the related viral strain bovine papillomavirus type 1, the precise placement of the recognition helices is significantly different. Additionally, the charge distribution on the DNA-binding surfaces of the two proteins varies; HPV-16 E2 has a much less electropositive surface. HPV-16 E2 is thus less able to utilize charge neutralization of the phosphate groups on DNA to induce bending. These results correlate well with previous solution studies that showed decreased affinity between HPV-16 E2 and flexible DNA target sequences, and enhanced affinity towards A-tract-containing, pre-bent sequences. In summary, the crystal structure of the HPV-16 E2 DNA-binding domain shows that the protein presents a stereo-chemically and electrostatically unique surface to DNA, characteristics that can contribute to its mechanism of DNA target discrimination.
About this Structure
1BY9 is a Single protein structure of sequence from Human papillomavirus type 16. Full crystallographic information is available from OCA.
Reference
Crystal structure of the E2 DNA-binding domain from human papillomavirus type 16: implications for its DNA binding-site selection mechanism., Hegde RS, Androphy EJ, J Mol Biol. 1998 Dec 18;284(5):1479-89. PMID:9878365
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