1byr
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= NUC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | |GENE= NUC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1bys|1BYS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1byr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byr OCA], [http://www.ebi.ac.uk/pdbsum/1byr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1byr RCSB]</span> | ||
}} | }} | ||
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[[Category: Stuckey, J A.]] | [[Category: Stuckey, J A.]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
| - | [[Category: phosphodiesterase]] | + | [[Category: phosphodiesterase,]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:11:06 2008'' |
Revision as of 16:11, 30 March 2008
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| , resolution 2.0Å | |||||||
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| Sites: | |||||||
| Gene: | NUC (Salmonella typhimurium) | ||||||
| Related: | 1BYS
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A PHOSPHOLIPASE D FAMILY MEMBER, NUC FROM SALMONELLA TYPHIMURIUM
Overview
The first crystal structure of a phospholipase D (PLD) family member has been determined at 2.0 A resolution. The PLD superfamily is defined by a common sequence motif, HxK(x)4D(x)6GSxN, and includes enzymes involved in signal transduction, lipid biosynthesis, endonucleases and open reading frames in pathogenic viruses and bacteria. The crystal structure suggests that residues from two sequence motifs form a single active site. A histidine residue from one motif acts as a nucleophile in the catalytic mechanism, forming a phosphoenzyme intermediate, whereas a histidine residue from the other motif appears to function as a general acid in the cleavage of the phosphodiester bond. The structure suggests that the conserved lysine residues are involved in phosphate binding. Large-scale genomic sequencing revealed that there are many PLD family members. Our results suggest that all of these proteins may possess a common structure and catalytic mechanism.
About this Structure
1BYR is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Crystal structure of a phospholipase D family member., Stuckey JA, Dixon JE, Nat Struct Biol. 1999 Mar;6(3):278-84. PMID:10074947
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